Re: [ccp4bb] Maltose binding protein as a tag

Dear Kostas There is a chance your protein is not properly folded by is solubilized by the large MBP tag, and this may be the reason for aggregation. Bostjan --- Bostjan Kobe NHMRC Research Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Mol

Re: [ccp4bb] database of crystallization condition

Hena I agree with the responses so far, but I think It may not be a complete waste of time looking at the crystallization conditions for similar proteins, you may find a common additive for example and there may be a functional reason for this being required in crystallization. Bostjan --- Bos

Re: [ccp4bb] ctruncate bug?

Hi Randy, So I've been playing around with equations myself, and I have some alternative results. As I understand your Mathematica stuff, you are using the data model: ip = ij + ib' ib where ip is the measured peak (before any background correction), and ij is a random sample from the true

[ccp4bb] Course “Structure and Function of Membrane Proteins- a multidisciplinary approach”, Portugal, 5-8- November

Dear colleagues, We would like to announce the 4-day course “Structure and Function of Membrane Proteins- a multidisciplinary approach”, to be held at ITQB, Oeiras (nearby Lisbon), Portugal, from 5 to 8 November 2013. The course will address several techniques for the structural and function

Re: [ccp4bb] Maltose binding protein as a tag

Dear Kostas, MBP by itself is a monomer. In most of our cases using it as a fusion tag, the yield of a target protein increases drastically (i.e. 5 to >20 folds more). However, we have seen in a couple of cases that the target proteins strongly interact with the MBP tag, which causes oligomeriz

Re: [ccp4bb] database of crystallization condition

Hena, I think this notion of fold families having similar crystallization conditions has been kicking around since the 80's at least. I seem to recall Gary Gilliland presenting a fairly comprehensive and well controlled study for myoglobins and showing some correlation of crystallization condit

Re: [ccp4bb] database of crystallization condition

Dear Hena, The BMCD might be a resource worth investigating: http://xpdb.nist.gov:8060/BMCD4/index.faces Although there are claims that structurally similar proteins may crystallise under similar conditions (the existence of directed screens -such as the Jena Biosciences 'Kinase' screen, you

Re: [ccp4bb] database of crystallization condition

On Wed, 24 Jul 2013 16:36:17 +0200, Hena Dutta wrote: Hi, Can anyone tell, if there is any database containing the crystallization conditions of published structures? I want to see the conditions people have used for those proteins having some structural similarity. Any suggestion would be appr

[ccp4bb] database of crystallization condition

Hi, Can anyone tell, if there is any database containing the crystallization conditions of published structures? I want to see the conditions people have used for those proteins having some structural similarity. Any suggestion would be appreciated. Regards... Hena

Re: [ccp4bb] Research Specialist in X-ray Crystallography position available at the BWH - No Longer Available

Dear all, This position is no longer available because it has been fulfilled. Posted on behalf of Richard S. Blumberg, MD. Best, Rosa

[ccp4bb] Maltose binding protein as a tag

Dear all, I would like to ask if someone has experience with maltose binding protein (MBP) as a tag. My protein fused to MBP seems to form oligomers that I find difficult to prevent and I was wondering if mbp behaves similar to gst and may also be prone to dimerisation/oligomerisation Many thanks

Re: [ccp4bb] Off topic - PEG smear availability

All PEGS are "smears" (technically speaking). But the ones you're asking about: they're dead easy to make, just toss together a bunch of 50% solutions of the individual components in whatever ratio you think makes sense. On 24/07/2013 11:36, AFL wrote: Dear All, I'm looking for a commer

[ccp4bb] Off topic - PEG smear availability

Dear All, I'm looking for a commercial suppliers of a PEG smear. Best regards, Andrzej Lyskowski -- Dr. Andrzej Lyskowski Core Facility Structural Biology Enzyme Development and Analytics ACIB - Austrian Centre of Industrial Biotechnology +43 316 873 9301 tel +43 316 873 9302

Re: [ccp4bb] which space group?!?

Dear Jürgen, dear Katherine, Sorry for a late reaction. In our article in Acta Cryst., 2009, D65, 1283-1291 (Section 4) we show an accurate distribution of the most frequent R, Rfree, Rfree-R as a practically linear function of the Log(resolution) , and not that of the resolution itself, and

[ccp4bb] AW: [ccp4bb] Where to cut the data in this medium resolution dataset

Dear Stefan, Did you have a look at the NCS related helices? To me it looks like your NCS restraints on B-factors are too strong, or not valid for your crystal packing. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Stefan G