Hi Joel,
You can just replace your current 'refmac5' and 'libcheck' files. Perhaps after
making a backup. They are in the 'bin' directory of your CCP4 installation.
The easiest way to find them is using 'which refmac5'.
HtH,
Robbie
Verzonden met mijn Windows Phone
__
Thanks Jim and Bert for all your suggestions. I plan to increase time
and/or amount of DDM and see what happens before switching detergents. I'll
keep the suggestions about other detergents and 96-detergent screen in the
back of my mind.
Bert, I noted your suggestion of also using a larger amount
I could add that even for membrane proteins that may not be stable in eg DM
after purification, DM may still be a viable option for extraction since there
will be large amounts of stabilising lipids present. Just make sure you change
to a milder detergent for the post-extraction steps.
Bert
___
Hi Raji,
the amount of detergent used is more important than the concentration. The more
cells you have, the more detergent is required. To give you an idea, we tend to
use 100 ml of 1% detergent (=1 g) for 12 liters of cells (Ecoli) of OD600 1.
This is probably on the low side; if cost weren't
Raji,
First, I would suggest increasing the solubilization time by attempting
solubilization overnight at 4 degrees. I would also suggest a few more
detergent choices. In addition to DDM, my other favories are OG and
Elugent (a mixture of several detergents) for extraction from *E.
coli* membran
Hi folks,
I am wanting to ask for some linux help as I want to try a newer version of
refmac (5.8) to test it against a refinement problem I have. My problem is that
I rarely use linux these days and I'm at a loss as to how to run the latest
version as my knowledge of linux has disappeared.
I
Dear BBers,
Sorry for the non-ccp4 post.
I'd like to hear tips and suggestions from the membrane protein folks in
the community.
I am currently purifying two different membrane proteins expressed in E.
coli. While I am able to extract practically all of my first protein from
the cell membrane in
Dear All,
We are currently seeking one postdoctoral fellow to work in the Guo laboratory
in the Department of Cancer Biology at the Scripps Florida campus of TSRI. The
lab is focused on the structural and cell biology of aminoacyl-tRNA syntheses
and their related pathways.
The position is full
Sadly not, we are dropping support for 32-bit Macs. You can still install
32-bit CCP4 6.3.0 from dmg.
Sorry -- Eugene
On 10 Jul 2013, at 16:04, hari jayaram wrote:
> Hi ,
> I am trying to use the CCP4 package manager on my 32 bit OSX 10.6.8 running
> laptop and it complains
>
> "/bin/sh: Volu
Hi ,
I am trying to use the CCP4 package manager on my 32 bit OSX 10.6.8 running
laptop and it complains
"/bin/sh: Volume/setup/.pm/Setup.app/Contents/MacOS/Setup: Bad CPU type in
executable.
Is there a 32 bit package manager available...
Thanks
Hari
<>
If you want to import integrated files from XDS into Pointless and Aimless, you
should use Pointless to do the conversion rather than the older program Combat.
This is what is done if you use the ccp4i interface task "Symmetry, Scale,
Merge (Aimless)"
Combat wrongly fills in the FRACTIONCALC co
POST-DOCTORAL POSITION IN STRUCTURAL BIOLOGY (PARIS, FRANCE)
.
A postdoctoral position is available in the group of Dr. Carine
Tisne at the Laboratory of Biological Crystallography and NMR of the
University Paris Descartes, jointly with the group of Dr. Ciaran
Condon at the Laborat
12 matches
Mail list logo
