Dear All,
If you are interested in learning more about microseeding techniques,
please join TTP Labtech’s June 18th webinar with Dr Alexey Rak, Sanofi R&D,
discussing high throughput seeding for the crystallization of small
molecules-protein complexes and antibody-antigen complexes. **
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Jeff,
thanks - I can see the same equation and cutoff applied in ctruncate
source.Here is the relevant part of the code
// Bayesian statistics tells us to modify I/sigma by
subtracting off sigma/S
// where S is the mean intensity in the resolution shell
h = I/sigm
Hi Ed,
I'm not directly familiar with the ctruncate implementation of French and
Wilson, but from the implementation that I put into Phenix (based on the
original F&W paper) I can tell you that any reflection where (I/sigI) -
(sigI/mean_intensity) is less than a defined cutoff (in our case -4.0),
I noticed something strange when processing a dataset with imosflm. The
final output ctruncate_etc.mtz, contains IMEAN and F columns, which
should be the conversion according to French&Wilson. Problem is that
IMEAN has no missing values (100% complete) while F has about 1500
missing (~97% complet
*Dear Abhinav Kumar
*
*thanks for kind suggestions
*
*
I have tried as follow.
1. You should try to identify the correct space group first.
*integration in p21 given the following statics in pointless
*
* Alternative reindexing Lklhd CC R(E^2) Number Cell_deviation*
* [h,
Dear Peter,
i am planning to produce a new pdb2ins containing several improvements,
but until this is ready please continue to use the old shelxpro.
Best wishes, George
On 17.06.2013 16:57, Peter Moody wrote:
At the risk of (more) people pointing at me and laughing...
I used to use SHELXPRO
Hi Pramod,
1. You should try to identify the correct space group first. Did you
integrate in p1 and run pointless?
2. A template with 31% identity is not a great model. The number of
molecules in ASU will affect your chances of success. Hopefully it's not
large. wrfac of 0.6 and Rfree of 0.5 s
Dear group
I have a crystal data diffracted around 2.9 A*,
during the data reduction HKL2000 not convincingly showed the space group
(indexed in lower symmetry p1), while the mosflm given C-centered
Orthorhombic, and again with little play around HKL2000 given CO, now the
model for molecular repl
We ran into the same issue. After checking the shelx site to see
if shelxpro was depreciated, I found the following in the FAQ
http://shelx.uni-ac.gwdg.de/SHELX/faq.php
Q27: How do I set up the first .ins file for SHELXL?
A: You will have to use the 'I' option in SHELXPRO (part of SHELX-97) u
At the risk of (more) people pointing at me and laughing...
I used to use SHELXPRO to get my .ins files sorted for SHELX, but that
seems to have gone.
How is it done now?
I want to do a full-matrix refinement to get ESUs on some (specific) atoms
and as far as I can remember SHELXL is the best wa
Dear Faisal,
I might be missing something, but why not ask the annotator instead of the
bulletin board? He should know which value is meant. For me, a sigma belongs to
a set of observations or parameters and without any further information one can
only guess (as Ed just did) which set. My guess
The only thing that seems to make sense is bonds rmsd - but you should
ask the annotator for specifics directly. If it is bonds rmsd, this has
been discussed many times - just google "rmsd bonds ccp4bb" and look for
most recent entries.
On Mon, 2013-06-17 at 12:11 +0530, Faisal Tarique wrote:
> D
A three-years PhD fellowship is available at the Laboratoire de Chimie des
Processus Biologiques, Collège de France, CNRS, Paris, France starting October
2013.
We are seeking an independent and highly motivated PhD student for protein
expression and structural studies on RNase Y, an essential m
Dear All,
I'd like to draw your attention to the following exciting opportunity at
Diamond Light Source for a Beamline Scientist to assist in designing and
building the new VMX submicron MX beam line that is currently in the early
stages of design. The beam line raises many challenges and as suc
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