Dear all
During PDB deposition the annotator me to verify and review the sigma value
in the structure file which in my case was -0.03. I have some basic queries
and request you all to please answer them. My first question is, what
actually is a "sigma value" of a structure file. 2nd) where the val
Hi Supratim,
if both the crystal and Mass spec tell you that you have 6k and 13k fragments,
proteolysis definitively took place. There are several possible explanations
for the phenomenom you observe:
-time: processes that do not occur during the few hours of a normal biochemical
experiments,
Hi
i have setup a crystallization of a complex formed by two different
proteins of molecular weights 53kD and 13kD. During purification of this
complex there was slight degradation band of 53KD protein as observed from
SDS PAGE but it did not effect complex formation. Crystals appeared after 8
mont
Disappearance of crystals is fairly common and not specific to DDM or indeed
any detergent as it also occurs for soluble proteins. Even after reaching
"equilibrium" conditions still change (evaporation, instability of components,
proteolysis, etc). The inverse also happens, that is formation of
