>Many NMR structures are more modelled than experimentally determined,
>the number of independent experimental
>data can be quite small. But the good news is that force fields and
>modelling software are improving.
>George
The quality of NMR structures/models depend heavily on the number of
exp
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The title of my PhD thesis was "NMR of inorganic hydrides" but I soon
realized that I was out of my depth with
the theory so switched to something easier to understand (gas phase
electron diffraction). However this involved
taking the (somewhat dangerous) samples by train to Durward
Cruickshank
Hi Theresa,
Per your question about determination of membrane proteins - solution NMR is
quite capable of delivering structures of proteins in the presence of
detergents, such as the KcsA channel (see
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242490/). You should note, though,
that many memb
Just to add some more information on the issue ... Take also a look at the
following article:
"Does NMR mean "not for molecular replacement"? Using NMR-based search models
to solve protein crystal structures"
Chen YW, Dodson EJ, Kleywegt GJ.
Structure (2000) 8, 213-220
Martin
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On Sunday, 09 June 2013, Theresa Hsu wrote:
> Dear all
>
> A question for the cross-trained members of this forum - for small sized
> proteins, is NMR better than crystallography in terms of data collection
> (having crystals in the first place) and data processing? How about membrane
> protein
I would agree with Mark.
It would be also good to state that neither of us is cross-trained, but are
one-trick dogs as far as NMR vs X-rays goes.
Still, I think that if you get any protein in good amounts, try to crystallize
it (there are even good facilities for that these days,
and funding t
Dear Theresa,
it depends what you consider as small. 50 residues are easy to do with NMR
but at 200 it will take some time (maybe 1-2 years to complete an
assignment and structure calculations). There are several difficulties in
NMR e.g. to do the correct alignment of monomers in oligomeric complex
Just to add on that, regarding membrane proteins: I noticed some recent
publications on solid-state NMR for membrane proteins, not sure about solution
NMR for this class.
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa
Well, if you do NMR you avoid the possible bottlenecks of having to obtain
well-diffracting crystals, and having to phase the protein (i.e. obtain SeMet
protein crystals or suitable heavy atom derivatives; or a suitable MR model).
But instead, you'll need to prepare labelled protein (15N and/or 1
Dear all
A question for the cross-trained members of this forum - for small sized
proteins, is NMR better than crystallography in terms of data collection
(having crystals in the first place) and data processing? How about membrane
proteins?
I would appreciate replies to the board, instead of
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