Not surprising since I'm sure they are both based on the Crick-Magdoff
equation (http://dx.doi.org/10.1107/S0365110X56002552) or the very
similar Hendrickson-Teeter equation (http://dx.doi.org/10.1038/290107a0).
Practically important thing is the signal-to-noise ratio. Roughly
speaking, to me
Similar result. Taking all defaults except #atoms, amt protein:
kDa #res #sites BjvtRatio
protein of interest:
LBL: 469 12 0.0153 (4 e-)
Ruppweb 427312 0.016(edge + 1 ev)
Ruppweb 4273
I put together a little jiffy for feasibility of MAD experiments here:
http://bl831.als.lbl.gov/xtalsize.html
It does not calculate the f" value for you, but in general f" is ~4
electrons for K edges and ~10 electrons for L edges. Crossec can give
you more accurate values than this as long as
On Saturday, 01 June 2013, Edward A. Berry wrote:
> Is Ethan Merritt's anomalous scattering page at:
> http://www.bmsc.washington.edu/scatter/
> down or moved, or the firewall I'm behind is blocking it?
The UW, in its infinite wisdom, scheduled a power outage
today so that they could replace the
Maybe I was a bit hasty- the Hb example gives only 2.4% difference;
2.0 at CuKa, if I'm interpreting the output correctly.
Maybe in the F. transform the random noise averages out and the signal
rises above.
But the Hb xtal probably diffracts really well with R-merge 3 or 4 in
the low resolution sh
How does that compare to something that readily works with Fe, such as horse
hemoglobin on a home lab copper X-ray system with 2 Fe in 291 residues in the
asymmetric unit?
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Edward A. Berry
[be
That's what I wanted. Results are not promising- Bijvoet differences
would be less than 2% at peak, and this is not lysozyme.
Thanks, all,
eab
Mooers, Blaine H.M. (HSC) wrote:
Bernhard Rupp has a calculator of Bijvoet ratios:
http://www.ruppweb.org/new_comp/anomalous_scattering.htm
Blaine Mo
Bernhard Rupp has a calculator of Bijvoet ratios:
http://www.ruppweb.org/new_comp/anomalous_scattering.htm
Blaine Mooers
Assistant Professor
Director Macromolecular Crystallography Lab
Member Stephenson Cancer Center
Department of Biochemistry and Molecular Biology
University of Oklahoma Health
Hmm- looks like crosssec calculates F' and F" (and cros-section).
I guess you're thinking of the "x-ray edge" page on the same server.
Ive got local copies and graphs of that in an excel spreadsheet.
I was thinking about the page where you enter your protein's
molecular weight, number and type of
seems to be down sorry.
you should be able to use crossec from ccp4
Jürgen
On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote:
Is Ethan Merritt's anomalous scattering page at:
http://www.bmsc.washington.edu/scatter/
down or moved, or the firewall I'm behind is blocking it?
I want to check feasib
Is Ethan Merritt's anomalous scattering page at:
http://www.bmsc.washington.edu/scatter/
down or moved, or the firewall I'm behind is blocking it?
I want to check feasibility of a native-iron MAD experiment,
and I'm not very good at math.
thanks,
eab
David,
Do you know of a program to make accurate braille representations of an
electron density map :)
That would be cool.
Maybe in the future [See:
http://www.wired.com/wiredscience/2012/08/smart-fingertips-virtual-senses/
http://www.ethlife.ethz.ch/archive_articles/100816_virtuelle_realita
How about Braille for those who are blind to all colours?
--
===
All Things Serve the Beam
===
David J. Schuller
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