Re: [ccp4bb] how to make the crystal thicker

I think there are some mitegen loops that are flat. They will probably be useful for your crystals given what you say about the 2.2 Å anisotropic diffraction, which is probably caused by deformation of your plates in "conventional" loops. Some others on the list may have a more specific recomme

Re: [ccp4bb] how to make the crystal thicker

Instead of making the crystal thicker, why don't you make the beamsize smaller ? Aka microfocus If this is your cryo-condition then I would work on it a bit more or change the drop ratio of protein:reservoir so that you can use a higher PEG3350 concentration in your reservoir. I would aim for >2

[ccp4bb] how to make the crystal thicker

Dear professors, I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350, however, it is very thin. From one side, the diffraction is perfect, about 2.2A, but from the other side, diffraction is too bad, the spots look like a thread! Process cannot be done by HKL2000. As t

Re: [ccp4bb] showing electron density on an ipad

Dave, We have now added a more direct and convenient way of viewing and contouring electron density maps on Android tablets and iPad using iMolview . To do this: 1. Download iMolview from iTunes

[ccp4bb] use only companies that you know to purchase chemicals

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[ccp4bb] Invitation to Participate in a NSF-funded workshop/conference

Invitation to Participate in a NSF-funded workshop/conference Dear Colleagues in Structural Biology, Recently we have received a NSF grant helping to build the community networks and find solutions for the challenging problems. We have also discussed with the program officer of US National Scie

Re: [ccp4bb] Improve diffraction ...any ideas?

Hi Urmi, When you say "antibody" you mean Fab fragments? If so, bear in mind that Fab fragments can be quite flexible about the region inbetween the variable and constant domains, which may be detrimental to the quality of your crystals ... in this case, further to the advice of others on here, you

[ccp4bb] Dimple on windows?

Is there any way to install the dimple script on my windows CCP4i installation? --Björn __ This e-mail may contain confidential information proprietary to Karo Bio AB and is meant for the intended addressee(s) only. Any unauth

Re: [ccp4bb] AW: [ccp4bb] Short contact between symmetry equivalents

Dear Sir, Ok Sir. I will check for other possible orientations. Thank you all for the Help.. Regards Kavya > Deav Kavya, > > The acetate is probably conformationally disordered. I would examine the > electron density maps to see if there might be an alternative orientation. > If so, you could m