Dear Greg,
The following might work:
set cylinder_shader_ff_workaround, on
Best regards,
Takanori Nakane
Hi,
We had a massive phage infection at the SGC in 2004; all our washing and
sterilization efforts could only solve the problem temporarily. I then
recovered a phage-resistant subclone of BL21(DE3), and prepared derivative
strains with pRARE2 (the tRNA plasmid in Rosetta2) or other plasmids. We
Dear Aidong,
Some thoughts...
Are you sure the problem is actually phage-related? I ask because you are still
having problems using phage-resistant stains...
Acid-washing your glassware, and a long-dry autoclave sterilisation (as
previously suggested) should really do the trick. A good *manu
Hi Aidong,
In our lab we are working with phages and sometimes we have the same
problem. But usually, the contamination is with other phages, not with the
ones we are growing. I can give you some ideas we tried during the last
year:
- Are you sure that nobody is growing phages nearby? In that case
Dear Aidong,
we never had that problem but I heard that only long dry(!) heat
treatment of glassware destroys the phages.
No need to say that you have to do it with ALL your glassware.
Good luck,
Matthias
-
Dr. Matthias Zebisch
Division of Structural B
Many thanks for your rapid inputs.
We used to make glyerol stocks but now we do not since the protein
expression is not stable. Our lab is about 10 students, who have to
make their own proteins so our three large-scale shakers are very
crowded every day. We did try a formaldehyde gas trea
Hi All,
Really sorry for my non-CCP4 related question. Our lab mainly uses
bacterial cultures to produce targets proteins for crystallizations.
However, we have been struggling with phage infections to our
bacterial cultures for a quite long time. To control its devastating
effects, we h
