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Dear Yuri,
If you have access to mass spec, this should be a straight forward experiment.
Find the
reference here.
h
Does that CA have a metal center (I think they all do)? If so, doesn't the
citrate compete for the metal?
JPK
On Mon, Feb 4, 2013 at 2:25 PM, Roger Rowlett wrote:
> Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium
> citrate/0.1 M TrisCl pH 8.5 at 10 mg/mL protein concent
Couldn't you just run reducing/non-reducing SDS-PAGE lanes and see the
difference?
JPK
On Wed, Feb 6, 2013 at 11:10 AM, Yuri Pompeu wrote:
> Dear All,
> I am trying to probe the existence of a disulfide bond on the surface of
> my protein.
> I have attempted Ellman´s and my results were not as
Hi,
to my knowledge, Ellman's reagent detects free thiols only. (Or am I
wrong?) In contrast, with the method of Thannhauser (Thannhauser [1987],
Methods Enzymol. 143, 115-9) you can determine the total amount of thiols
(free and in disulfide bonds). Any difference between the two methods
sho
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I never done such experiments but N-Ethylmaleimide labeling and Mass-Spec could
be the solution.
Le 6 févr. 2013 à 17:10, Yuri Pompeu a écrit :
> Dear All,
> I am trying to probe the existence of a disulfide bond on the surface of my
> protein.
> I have attempted Ellman´s and my results were n
Dear All,
I am trying to probe the existence of a disulfide bond on the surface of my
protein.
I have attempted Ellman´s and my results were not as clear as I would have
hoped for.
I am not a sulfur/cysteine chemist and would appreciate the advice on what
experiments to try!
Thanks a bunch
YAP
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Hello Xavier,
if you look at the downloaded file, you'll notice the source of the
error: The pdbe download link has changed, i.e. this is not a
PDB-file. You'll have to download the PDB file manually until it is
fixed in the latest coot-code.
Cheers,
Hi Tim
I've looked at these images and they differ from the normal 6M images
("miniCBF") in that they are a stab at writing "fullCBF", i.e. with
imgCIF style data content - unfortunately, there are a few syntax
errors which need fixing before programs that use the header
information (like
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Dear Jose,
it is odd the software should read 6M but not 0.3M Pilatus files - the
format is probably the same, only the dimensions would differ - at
least that's my guess.
While you are waiting you might start processing your data with XDS,
which is w
Dear all,
sorry for the semi-off-topic but I'm trying to help convert some diffraction
images and this seemed like a good place to ask. We are trying to process some
images collected on a Pilatus 300K with the Crysalis Pro software (small
molecule) but it seems to only be able to read Pilatus 6M
Just to add some more possibilities:
- You can download maps from EDS or models and maps from PDB_REDO straight into
CCP4mg.
- You can download PDB_REDO maps and models into PyMOL using this plugin
(http://www.cmbi.ru.nl/pdb_redo/pymol.html) for which we should thank Ed
Pozharski. Note that th
Gentle reminder of the following openings at SGC Oxford (deadline 7th
February noon). Thanks.
On 14 January 2013 17:51, Wyatt W. Yue wrote:
> Dear all,
>
> We are seeking two post-doc scientists in the Metabolic & Rare Diseases
> group at the Structural Genomics Consortium (SGC), University of O
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