Dear CCP4ers,
Recently I am trying to process a dataset with XDS program, but during the
intergrate and scale procedure it always ended with the following warning:
!!! ERROR !!! ASSERT VIOLATION IN "RESTHKL"
**
Sucrose co-crystallized in lysozyme is wonderful. I think a soak will crack the
crystals, but sometimes not.
Here's a pic of electron density from an orthorhombic crystal of lysozyme
solved in the recent 2012 CSHL X-ray Methods in Structural Biology course:
http://i46.tinypic.com/2e4xk7k.jpg
before modelling a long side-chain in non-existing or dubious density,
also make sure it is really there in the protein by sequencing your
expression plasmid. Your arginine (for example) may in fact be a
serine or glycine...databases are not 100% accurate and neither is PCR
if it was used i
OK, here we go again.
This has been argued ad nauseam, see for example
http://www.dl.ac.uk/list-archive-public/ccp4bb/msg19738.html
or
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg20268.html
(hard to believe we have gone more than a year without another version
of "what to do with diso
Hi Guys,
I'm dealing with the same issue with a couple of structures at the moment. Matt
you said that you like to leave the side chains in a plausible conformation and
let refinement deal with the problem. Generally when I try this their backbone
geometry gets all bent out of shape in refineme
