Dear users,
Has there been any instances where there is an
anion-pi interaction in proteina. I found a density
that could accommodate an acetate ion just above
the aromatic ring of the tyrosine residue in the
protein I am working on. I just came across an article
regarding the anion-pi interaction
Hi,
Try glutaraldehyde crosslinking of peaks you are obtaining from SEC
individually and see if it gives you some idea.
Best wishes
Gauri
Lucas, if your crystals diffract worse at room temp than cryoprotected, it
looks like there may be radiation damage at room temperature, so you may
need to cryoprotect. The change in osmotic pressure may be too much for
your crystals when you're cryoprotecting the crystals by a sudden dunk.
Also, d
Multi-Angle Light Scattering (MALS) coupled with an HPLC column attached to
an analytical SEC column is my standard "go-to" method for determining
molecular weights/oligomeric states of peaks from SEC runs. I've only ever
used the detectors from Wyatt (
http://www.wyatt.com/solutions/hardware/hard
Hello all,
I am working with a protein that is probably a hexamer based on homology
with other proteins but when I ran it on an analytical size gel filtration
column, I see multiple peaks. I would like to determine the exact
oligomerization state of the mixture and have considered blue native gel
Hi,
I just upgraded to the newest CCP4 version 6.3 and noticed that libcheck
which coot uses to produce restraints from a SMILES string produces garbled
coordinates in ccp4 version 6.3 , but the same SMILES string works just
fine with CCP4 version 6.2.
I tried to get it to fail on public molecule
Dear all,
Thank you very much for your cooperation.
Regards,
Sudipta.
Sudipta Bhattacharyya.
Senior Research Fellow,
Department of Biotechnology,
Indian Institute of Technology Kharagpur, India.
