[ccp4bb] Crystallisation video

Hi, Does anybody know of a video of setting up crystallisation of HEWL or other protein straightforward to crystallise (ie setting up "manually")? Such a setup has the potential even to be popular in schools, I think. I mean I want the experiment shown, and not just a lecture on protein cryst

Re: [ccp4bb] pseudo translational symmetry

Brilliant illustration - even in 3D! eleanor On 3 Aug 2012, at 14:01, Laurie Betts wrote: > I couldn't resist this shot. It might not make the cut.but > >

Re: [ccp4bb] pseudo translational symmetry

"Some people might squawk at 2MB attachments on a BB..." Surely that is bark :)

Re: [ccp4bb] pseudo translational symmetry

One way to address this would be to provide a link to the image on the web, rather than including it as an attachment. On 08/03/12 14:04, Francis E Reyes wrote: Some people might squawk at 2MB attachments on a BB... But that made my day.. Thanks! On Aug 3, 2012, at 6:01 AM, Laurie Betts w

[ccp4bb] SCALA bugs in CCP4 6.3.0?

In the Windows version of the new CCP4 release SCALA produces a summary table (same in the full text) that contains only some rudimentary data, such as Overall InnerShell OuterShell Low resolution li

Re: [ccp4bb] pseudo translational symmetry

Some people might squawk at 2MB attachments on a BB... But that made my day.. Thanks! On Aug 3, 2012, at 6:01 AM, Laurie Betts wrote: > I couldn't resist this shot. It might not make the cut.but > >

Re: [ccp4bb] pseudo translational symmetry

What symmetry operator is that towel indicating? Must be 24-fold rotational NCS? JPK On Fri, Aug 3, 2012 at 8:43 AM, Bosch, Juergen wrote: > Thank Laurie, > > what's that domain dangling off your DOG-domain ? And have you checked if > the off-origin peak is indeed the same height as the origin

[ccp4bb] 回复： [ccp4bb] asking for a reference for cacodylate decomposition in protein crystals upon X-ray exposure

Dear Tanya, We have published a paper related to this topics on PloS One last year (see below), and we have not generally encountered "crystal degradation pattern upon X-ray exposure". "Get phases from arsenic anomalous scattering: de novo SAD phasing of two protein structures crystallized in

Re: [ccp4bb] pseudo translational symmetry

Thank Laurie, what's that domain dangling off your DOG-domain ? And have you checked if the off-origin peak is indeed the same height as the origin peak ? May I include your slide in my class ? Jürgen On Aug 3, 2012, at 9:01 AM, Laurie Betts wrote: I couldn't resist this shot. It might not

[ccp4bb] Crystallization Robots

Dear Sam We have recently bought a Gryphon LCP to accommodate routine seeding experiments. We are actually using the LCP module to dispense the seed stocks. We had never encountered problems with calibration and our system is very reliable and running stable. I fully agree with Rogers’ statemen