Hi folks,
Sorry for the cross post / off topic question. I have just noticed a
"recurring/repeatable problem" when using the get monomer option in Coot. E.g.
when getting ILE (and a few others) but not ala, Coot freezes on a windows 7
machine (using 64-bit thingies). I am using 0.6.2 and there
Postdoctoral Position at ICR, London, UK
We seek to appoint a Post-doctoral Training Fellow to the Divisions of
Structural and Cancer Biology of the Institute of Cancer Research (ICR;
http://www.icr.ac.uk) in London, UK, to a new team led by Dr. Sebastian
Guettler. The successful applicant will
The HZB MX-team is seeking a beamline scientist to support the operations of
the three MX-beamlines at BESSY-II. The initial contract will run over a period
of three years. Payment will be on the basis of germans TVöD-Bund salary scheme.
HZB-MX is offering state-of-the-art MX-beamlines to the int
Hi Fengjuan,
It has been proposed that for a specific protein there are "hard precipitants"
and "soft precipitants". Hard ones precipitates the the protein effectively,
while soft ones may help stabilizing the protein. By playing with the two types
of precipitants, one may get better crystalliz
Hi,
The idea used by the Qiagen pre-screen might be relevant to your case:
http://www.qiagen.com/products/protein/crystallization/screeningproteincrystallizationconditions/prescreenassay.aspx
http://www.qiagen.com/literature/handbooks/literature.aspx?id=239
Zhijie
From: fengjuanlv12
Sent:
hi all,
I got a protein with good condition without precipitating in the purification
process.And the protein is stalbe at 4oC, but when i try crystallization
screens, it precipating in all the kit(with PEG), in the kit with high
concentration of salt ,the situation was better(but still no crys
Dear Michael,
the purpose of our paper (http://www.sciencemag.org/content/336/6084/1030) was
to develop and document methods that enable to check for yourself what the best
high resolution cutoff is - no rules of thumb are necessary!
However, repeated use of the "paired refinement technique" (3
