Dear All,
As I am practicing new in the crystallography, I am facing some difficulties in
refining the ligand bound structure. Protein I am working with has SG P212121,
it's a dimer. I fitted the ligand on the density with
COOT-->calculate-->Model/Fit/Refine-->Rotate/Translate Zone. Then I merg
Hi All,
We are trying to deal with a eukaryotic membrane protein that crystallizes
but, as usual, diffracts poorly so far. As it is heavily glycosylated, we
are considering enzymatic deglycosylation.
Does anyone know about a recombinant source of N-glycanase ; we would like
to prepare it ourselves
Hi
Could you please check:
1) If there is psedotranslation. It could be done by using sfcheck, molrep,
ctruncate or calculating patterson map and displaying using coot at 8-10 sigma
level (it is my favourite method for analysis of pseudo translations), whole
unit cell ( a bit bigger than whole
