Re: [ccp4bb] Off-topic: ELNs

Hi Seiji, I would recommend Labguru (www.labguru.com) which is cloud-based ELN and laboratory management solution. This tool encompass the management of experiment and projects, connects between experiments and individual samples, manages the different storage facilities down to the single tube and

Re: [ccp4bb] Off-topic: ELNs

Hi Seiji, A nice web base solution is the iLabber from Contur. It has been a popular option for many academic labs around the globe. http://www.contur.com/home/ Cheers, Francisco Sr. Product Manager Accelrys, Inc. -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.A

Re: [ccp4bb] Off-topic: ELNs

My lab uses http://biochemlabsolutions.com/ELN/ELN.html Best, Alexei. On 1/16/2012 5:03 PM, Sean Seaver wrote: Hi, off-topic question regarding electronic laboratory notebooks. Our lab is planning on moving from paper to digital record keeping and I was wondering which of the available ELN platf

Re: [ccp4bb] Off-topic: ELNs

>Hi, off-topic question regarding electronic laboratory notebooks. Our >lab is planning on moving from paper to digital record keeping and I >was wondering which of the available ELN platforms are being used by >the ccp4 community. >We are primarily a crystallography lab but we would also need so

[ccp4bb] Off-topic: ELNs

Hi, off-topic question regarding electronic laboratory notebooks. Our lab is planning on moving from paper to digital record keeping and I was wondering which of the available ELN platforms are being used by the ccp4 community. We are primarily a crystallography lab but we would also need some ve

[ccp4bb] postdoctoral position at the MRC LMB Cambridge

A post-doctoral position in AMPA glutamate receptor biology (AMPA-Rs) is available in Ingo Greger's lab at the MRC LMB, Cambridge. This post is funded by MRC-Technology and aims to develop AMPA-R modulators targeting the receptor's N-terminal domain (NTD). We seek an experienced crystallographer wi

[ccp4bb] Postdoctoral Position in Mechanistic Enzymology at the University of Iowa

Postdoctoral Position in Mechanistic Enzymology at the University of Iowa The Dey lab in the Department of Chemistry at the University of Iowa seeks to recruit an outstanding postdoctoral scientist with experience and/or interest in mechanistic enzymology. The successful candidate will be respon

Re: [ccp4bb] Off topic: His-tag purification

…alternatively, depending on what is the source of your protein, you might have to dialyze the (concentrated) sample before applying it to IMAC. Insect cell media, in particular, can be pretty good at stripping off Ni2+ from IMAC supports. HTH, Luca

[ccp4bb] La Caixa"/IRB Barcelona PhD fellowships Programme selection procedure

Dear colleagues, Please forward this information to students in your institutes who might be interested. L a Caixa"/IRB Barcelona International PhD Fellowship Programme Structural and computational biologists IRB Barcelona is an ind

[ccp4bb] AW: [ccp4bb] Off topic: His-tag purification

Hi Theresa, did you try to change the position of the His-Tag? Perhaps it is not exposed in your current construct and would work better at the N- or C-terminus. Greetings Moritz Metlitzky, Ph.D. Institute of Toxicology Tel.: 089-3187-2669 Fax: 089-3187-3449 www.helmholtz-muenchen.de/en/toxi/krap

Re: [ccp4bb] Off topic: His-tag purification

Hi Theresa, You could try lowering the pH down to 7.4 or 7. I have found that some proteins bind very poorly at pH8 and higher. Hope this helps. Cheers! --- Greg Costakes PhD Candidate Department of Structural Biolo

Re: [ccp4bb] Off topic: His-tag purification

-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Theresa, you might also try changing the resin. I have worked on a protein which would no bind to Ni-NTA (Qiagen) at all but could greatly be purified when using Ni-IDA (then Pharmacia) instead. The protein expressed to about 60mg/l LB in E. coli