Thank you for all the quick reply. Here are the additional information
about the crystal. The crystal was frozen and shot in synchrotron. The
collection was 1degree/frame. The space group and unit cell are P21
and a=70 b=165 c=170 alpha=90 beta=90.1 gamma=90. I processed the
data in P1 first (I di
The laboratories of Vanessa Ruta (The Rockefeller University) and Dr. Filippo
Mancia (Columbia University Medical Center) are looking for a talented and
highly motivated structural biologist for a postdoctoral position to work on a
collaborative project to determine the structure and function of
The Prehoda Lab at the University of Oregon has an opening for a postdoctoral
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will investigate the mechanisms of protein domain evolution, in part by
determining the structures of proteins from 600 million year old res
El 04/01/12 21:21, Artem Evdokimov escribió:
> Wow.
>
> Harsh interview criteria! It's a relief to know that I don't need a job,
> cause I wouldn't pass.
>
> Artem
>
Dear Artem,
You see, using one's creativity to by-pass a robotic application server
to make a few, direct, clear, concise questi
Wow.
Harsh interview criteria! It's a relief to know that I don't need a job,
cause I wouldn't pass.
Artem
On Jan 4, 2012 1:36 PM, "Sheriff, Steven" wrote:
> CONTACT ME ONLY IF YOU HAVE TECHNICAL DIFFICULTIES WITH THE LINK OR THE
> ONLINE APPLICATION PROCESS. DESCRIBE YOUR PROBLEM AND I WILL F
What are your cell constants and space group? It sounds to me you
misindexed and then artificially "twinned" your structure by
integrating/merging in too high of a symmetry. I've seen that happen for
primitive hexagonal which was actually C-centered monoclinic.
Also, in my experience this is more l
CONTACT ME ONLY IF YOU HAVE TECHNICAL DIFFICULTIES WITH THE LINK OR THE ONLINE
APPLICATION PROCESS. DESCRIBE YOUR PROBLEM AND I WILL FORWARD IT TO BMS HUMAN
RESOURCES. SENDING ANYTHING ELSE DIRECTLY TO ME WILL BE PERCEIVED AS AN
INABILITY TO FOLLOW SIMPLE DIRECTIONS AND CAUSE TO CONSIDER DISCARD
Dear All
for all those not lucky enough to attend the CCP4 Study Weekend on "Data
collection and processing" in Warwick there will be live streaming. This is at
the link http://extrplay.dl.ac.uk/ kicking off with the what's new session at
9:00am GMT. The main event starts at 11:00am GMT.
The
Need more info: how many degrees per frame? Also, on integration, do
various stats change over the 'sets?
JPK
On Wed, Jan 4, 2012 at 7:42 AM, Zhiyi Wei wrote:
> Dear all,
>
> I recently collected a dataset (~2000 frames) from a single crystal.
> If merge first 600 frames (sca1) or last 600 frame
Dear Tong,
I actually raised this point with the developers of Phaser a while back, at a
CCP4 meeting, as the wording and nomenclature can be a little confusing at
times.
Molecular replacement in Phaser can be done with a single structure, or an
ensemble or "group" of structures (all of which
Dear all,
I recently collected a dataset (~2000 frames) from a single crystal.
If merge first 600 frames (sca1) or last 600 frames (sca2), Rmerge
values from scalepack seem to be ok (~10%) though rejection ratios are
high (~5%). But if I merge all frames together, Rmerge value goes up
to ~20% and
Thank you for your reply!
But I still wonder the differences between "Add superimposed PDB file to the
ensemble" and "Add ensemble" in Phaser program and when to use the former.
If I want to search for a complex model, I often just click the "Add ensemble"
till completing the search model.
--
Add an ATOM AT THE POINTER - call to water I guess -write it to your
coordinate file, then redo the refinement - the green blob should disappeat.
Could that O pointing towards the grenn blob be flipped though? there is
a water in rather a strange position opposite, although the picture
might
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