Just you wait--he'll be back...won't he?
On Tue, Nov 29, 2011 at 11:11 PM, James Stroud wrote:
> With such a nice parting letter, we find it disheartening to let you go.
>
> James
>
> On Nov 29, 2011, at 7:44 PM, debanjan choudhuri wrote:
>
> To whom it may concern,
> I am writing this in reques
With such a nice parting letter, we find it disheartening to let you go.
James
On Nov 29, 2011, at 7:44 PM, debanjan choudhuri wrote:
> To whom it may concern,
> I am writing this in request to the cancellation of my subscription to CCP4BB
> mail.
> It was indeed a very rewarding and a knowledg
To whom it may concern,
I am writing this in request to the cancellation of my
subscription to CCP4BB mail.
It was indeed a very rewarding and a knowledgeable experience
to be a part of this family.
With regards
Debanjan Choudhuri
Dear Crystallographers,
does anyone have any nice examples/references of proteins which form
demonstrably physiologically-relevant oligomers in crystals, but which
do not appear to do so in solution? I am thinking particularly that
domains of membrane proteins which oligomerize primarily through t
Dear Crystallographers,
Thanks very much for all who responded to my request. Below is a
compiled list of the various ways to skin this crystallographic cat!
Jacob
-Moleman ca plot distance-CCP4's superpose-distance matrix analysis,
e.g. http://www.csb.yale.edu/userguides/datamanip/ddmp/ddmp_descri
0.5% octyl-thioglucopyranoside + lysozyme + DNAse in 30 mM TRIS pH 8.0 -
degassed/sparged and supplemented with whatever reducing agent you need.
Stir in cold for 30-40 minutes at a ratio of 3ml of solution to 1g of cell
pellet (wet).
Artem
On Tue, Nov 29, 2011 at 8:49 AM, Jim Spencer, Cellular a
Dear All,
Apologies for the off-topic question. I'm seeking suggestions on the best
way to achieve an effective, but not over-harsh, chemical lysis of E coli
expressing an oxygen-sensitive Fe-S protein. We need to lyse anaerobically
but do not have access to a sonicator that we can use in th
Christine, I have a couple more comments
First, if those crystals come back, I would certainly try microseeding into
*random *screens. You should be able to pick up new conditions that have a
little more salt in them, and are therefore stable regarding the movement
of water. (If you can't get th
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Hi CCP4 community.
While running the Phaser in the CCP4 GUI, It was terminated and giving the
error message.
#CCP4I TERMINATION STATUS 0 "child killed: segmentation violation"
#CCP4I VERSION CCP4Interface 2.0.6
CCP4 Version ccp4-6.1.13
Thanks in advance.
Sita Ram
Dear Christine,
I had guessed that you had more salt in the protein solution
than in the reservoir with a relatively low protein concentration.
The protein is in:
100mM Sodium Acetate pH 5.5 with 150mM NaCl at a protein concentration
of ~4.3mg/mL.
and the reservoir is:
(0.05M Calcium chlor
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Hash: SHA1
Dear Jacob,
Coot prints this information to the terminal, so you can start coot and
'tee' its output into a file.
Tim
On 11/28/2011 11:53 PM, Jacob Keller wrote:
> Let me refine my question (sorry for my lack of clarity): is there a
> program that w
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