Hi all,
I got a protein-DNA complex crystal,by running SDS-PAGE and nativePAGE,i prove
that the crystal contain both
protein and DNA.BUT it does not diffract. does anyone have suggestion to
improve the diffraction ability ?
Best wishes,
deng
Hi James,
I don't think the comment you referenced meant to imply "dark progression of
radiation damage. If I remember from the recent thread, it was to say that if
you can only collect few (3?) shots from one crystal before it's "too dead" and
you use 1st of these shots to devise the strategy,
Dear Rajesh,
Are you using the Openmotif library? If so, do you
have an XKeysymDB file installed?
In as shell, issue:
$ locate XKeysymDB
You may need to symlink it to /usr/X11R6/lib/X11/XKeysymDB if it is
elsewhere [1].
I suspect you're not the only one to have seen this [2].
I h
Mark's comment below reminded me of a quandary that is starting to
develop in the rad dam field. The idea of the "free radical cascade"
continuing to damage protein crystals even after the beam has been
turned off seems to have originated on page 253 of Blundell and Johnson
(1976), and I thi
Dear Tim,
Thanks. Your suggestion of adding to PATH works and its not completely
functional may be due to the Ximg/ssh or some thing to do with display.All
three windows opened but couldn't open any image as it didn't display in the
list of autoload window. Pattern shows *.0.
$adxv pin8_1_180
A source of confusion is that we are using two different definitions of
"origin". In the a graphics program sense (coot, pymol), the origin is always
at xyz coordinate (0, 0, 0). I think that's what Napo means by "present the
same cell and origin."
However, imagine a crystal upon which we pu
"one origin to rule them all" works in the reciprocal space
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Napoleão
Valadares [n...@if.sc.usp.br]
Sent: Sunday, November 20, 2011 10:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb]
Thank you all for the replies. Felix Frolow, Dan Leahy, Hans
Brandstetter, Boaz Shaanan and Tim Gruene you really helped a lot.
I think I understand it now, I always thought the "one ring to rule them
all" translated in the crystallography realms to "one origin to rule
them all". That probably
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Dear Napo,
to illustrate Felix Frolow's comment, take Solution-1 and the new data
to refmac5 and run 'rigid body refinement'. I would not be surprised if
you get a reasonable map with nearly the same R/Rfree as your MR
solution, which means that this
