Re: [ccp4bb] crystallization of synthetic peptides

Some HIV protease structures have been done using synthetic HIV protease (99 amino acid monomers). Look at J. Martin et al from UQ in Queensland. I believe this was done with Steve Kent. The protein contains some non-natural amino acids too. Hope this helps -Original Message- From: CCP

Re: [ccp4bb] effect of Izit dye within the crystal structure

I wouldn't be surprised if it is methylene blue. Jürgen On Nov 10, 2011, at 11:34 PM, adam andres wrote: Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this d

[ccp4bb] effect of Izit dye within the crystal structure

Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that presentthis dye? Cheers Adam Campos

[ccp4bb] Web Seminar: Home Lab SAD phasing with HKL-3000: From data collection to refined models in less than an hour

Dear colleagues, I would like to draw your attention to an upcoming free, educational webinar to be presented by Jim Pflugrath, Ph. D. titled "Home Lab SAD phasing with HKL-3000: From data collection to refined models in less than an hour." This interactive tutorial and webinar will demonstrate ho

Re: [ccp4bb] How to calculate the percent of the buried hydrohobic surface area in a protein with known structure

Jiyuan, I believe PISA will easily do this for you. C On Thu, Nov 10, 2011 at 5:10 PM, Ke, Jiyuan wrote: > Dear All, > > ** ** > > I have a protein that exists as a dimer in the crystal structure. I want > to calculate and compare the area of the buried hydrophobic core of a > monomer wi

[ccp4bb] How to calculate the percent of the buried hydrohobic surface area in a protein with known structure

Dear All, I have a protein that exists as a dimer in the crystal structure. I want to calculate and compare the area of the buried hydrophobic core of a monomer with that of a dimer? Does anyone know how to do this? Thanks in advance! Jiyuan Ke, Ph.D. Research Scientist Van Andel Research Inst

Re: [ccp4bb] crystallization of synthetic peptides

As Mark says, structure solution of smallish peptides is not usually as easy as one might expect. A number of the small (say up to 50 residue) peptides in the PDB were solved by direct methods, but these require native data to 1.2A or (preferably) better. If sulfur is present in the molecule, S

Re: [ccp4bb] crystallization of synthetic peptides

Hans, Most natural toxins from snakes, scorpions etc are 50+/-some peptides. And quite a few of those have been studied and crystallized (see pdb for a list). Having worked on one of these structures as a graduate student, I can share my experience: - Purification is harder than you would thi

[ccp4bb] crystallization of synthetic peptides

Dear crystallographers, Because of the low cost and speed of synthesizing 40- to 60-mer peptides, I wonder whether anyone has (good or bad) experiences crystalizing such peptides. In literature, I've found up to 34-mer synthetic coiled coils, but no other protein class. I can imagine that a protei

[ccp4bb] PhD and Post-doctoral Positions in Membrane Structural Biology at University of Freiburg

A post-doctoral position and a PhD position are open to study structure, mechanism and function of clinically relevant cation/proton antiporters and their integration in signalling processes. The project is part of the Collaborative Research Center 746 (http://www.sfb746.uni-freiburg.de) and is

Re: [ccp4bb] RFI on access to digital data

Dear Ashley, Excellent find! Within it I see that one of the UK agencies states:- vii. What resources will you require to deliver your (Data management and sharing) plan? Greetings, John On Wed, Nov 9, 2011 at 10:50 PM, Deacon, Ashley M. wrote: > I also found this interesting: > > http://www.d