Re: [ccp4bb] Phaser and Molrep gave different solutions

2011-07-20 Thread Francois Berenger
On 07/21/2011 01:45 PM, Hubing Lou wrote: Dear all, I am stuck in a molecular replacement case and looking for advices. I have been working on a protein-DNA complex structure. Data was processed by HKL2000 to 2.6Ang and some of the data statistics are shown below: Space group: P21, Unit Cell: 5

Re: [ccp4bb] CCP4 6.2.0 reading mtz files

2011-07-20 Thread Tom Peat
Hello All, It turns out that the /tmp directory being used by ccp4i had incorrect permissions, which gave rise to the problem. Thanks for the solution! Cheers, tom -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tom Peat Sent: Thursday, 21 July

[ccp4bb] CCP4 6.2.0 reading mtz files

2011-07-20 Thread Tom Peat
I took a quick look via Google and in the FAQ to see if there was anything on this topic, but didn't find the clue I was looking for. I just installed version 6.2.0 on a linux box running Centos 5.6 and I get error messages every time I try to read in a mtz file- unreadable. I check the file us

Re: [ccp4bb] Denzo/HKL2000 for Pilatus 6M detector

2011-07-20 Thread Graeme Winter
Dear Petr, Here at Diamond we have a version of HKL2000 which works for Pilatus detectors. Best wishes, Graeme On 20 July 2011 12:25, Petr Leiman wrote: > Dear all, > > What is the status of Denzo/HKL2000 availability/support for the Pilatus 6M > detector? > > Thank you, > > Petr

Re: [ccp4bb] Denzo/HKL2000 for Pilatus 6M detector

2011-07-20 Thread Soisson, Stephen M
We have a version of HKL200 that supports the Pilatus6M. That said, most here prefer XDS for processing. Steve -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Petr Leiman Sent: Wednesday, July 20, 2011 7:26 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [

Re: [ccp4bb] Denzo/HKL2000 for Pilatus 6M detector

2011-07-20 Thread Felix Frolow
Why not to ask The Lord of Rings? FF Quoting "Petr Leiman" : Dear all, What is the status of Denzo/HKL2000 availability/support for the Pilatus 6M detector? Thank you, Petr Dr Felix Frolow Professor of Structural Biology and Biotechnology Department of Molecular Microbiology and Biote

[ccp4bb] Denzo/HKL2000 for Pilatus 6M detector

2011-07-20 Thread Petr Leiman
Dear all, What is the status of Denzo/HKL2000 availability/support for the Pilatus 6M detector? Thank you, Petr

Re: [ccp4bb] Bypassing phase separation for nice crystals.

2011-07-20 Thread Hargreaves, David
Hi Timur, I've had a crystal which sounds similar to yours. When I came to freeze it noticed that it was not exactly solid more amorphous like treacle. Laughingly I went ahead and screened it anyway and was shocked to see diffraction to around 15Ang. I was lucky enough to have other crystals in

Re: [ccp4bb] Poor proline electron density

2011-07-20 Thread Herman . Schreuder
Dear Dolphin, That N- or C-terminal residues are not as well ordered as the rest of the protein happens very often and is nothing to be concerned about, especially if there is clear density at the 1 sigma level. Prolines are rigid, but can exist in cis- and trans-conformation. I have seen many ca

Re: [ccp4bb] Data from old tapes

2011-07-20 Thread Vellieux Frederic
Plenty of old drives at ESRF, for that purpose. If you don't find any other way, I could always read them there (the ESRF is "in my backyard") and burn the data to DVD for shipping (or upload to megaupload - but our gov't is hunting down people who use such services, they think megaupload and o