Hi Ruheng,
Curious to know what was in the crystallization cocktail, cryoprotectant?
Did you collect the dataset at a synchrotron?
Shiva
2011/7/4 ruheng
> Dear all,
>
> Recently we are working on an archaebacteria protein which was expressed
> and purified from *E.coli *by conventional proced
Mon. July 4th 2011
EBI
Good evening,
firstly, it could be something that came form the buffer/crystallisation
soup etc. What about phosphate?
Secondly, if you think it is a "natural" post-translation modification of
your specific protein this is gene/source depended. Check the uniprot
entry
Before ruling this a covalent modification, did you check if a sulfate or
some other tetrahedral ion (or a disordered acetate) fit the bill with
respect to distances to the neighboring atoms?
Artem
2011/7/4 ruheng
> Dear all,
>
> Recently we are working on an archaebacteria protein which was e
Looks like an anion, possibly sulfate or phosphate. What's in the
crystallization mix?
Roger Rowlett
On Jul 4, 2011 11:33 AM, "ruheng" wrote:
>
> Dear all,
>
> Recently we are working on an archaebacteria protein which was expressed
and purified from E.coli by conventional procedures. After we so
The distance to your modelled water seems to be in agreement with an
ionic interaction. What are the components of all buffers in the
crystallisation components ?
Fred.
ruheng wrote:
> Dear all,
>
> Recently we are working on an archaebacteria protein which was
> expressed and purified from /E.co
***
Research Officers in Dynamic Structural Science at the Research Complex
at Harwell and the University of Bath (2 posts, fixed term for 4 years)
***
Applica
This is a problem not properly addressed - if you generate your
FreeRflags in the highest possible Laue group for a system - eg P6/mmm
if you SG is trigonal, then add these to the lower symmetry reflection
list you are safe. It really should be done at the pointless stage but
it isnt..
Here i
Hi all,
I am worried about mixing working and free reflexions during TWIN refinement.
Is there any keyword in UNIQUEIFY to generate a R-free flag in the case of
twinning?
I mean in that way that the possible twinning operators are taken into account
for a following
refmac job using the TWIN k
Hi all,
I am worried about mixing working and free reflexions during TWIN refinement.
Is there any keyword in UNIQUEIFY to generate a R-free flag in the case of
twinning?
I mean in that way that the possible twinning operators are taken into account
for a following
refmac job using the TWIN k
Can you send a bit of the input file and the command script generated by
the GUI
Eleanor
On 07/02/2011 07:48 AM, Sudhir Kumar wrote:
Dear all
I am trying to convert a scaled file (scaled using Automar) to mtz butit is
showing follwoing error
:
#CCP4I TERMINATION STATUS 0 Error from script
/o
Hi All,
I am looking for a laptop computer that works well with stereo viewing on Coot,
Pymol, and Discovery Studio.
Nvidia 3Dvision webpage offers quite a few models but almost all of them in
market is 17" and thus too heavy to carry around.
Please, if any of you could suggest any model of
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