Dear all
I am trying to convert a scaled file (scaled using Automar) to mtz butit is
showing follwoing error
:
#CCP4I TERMINATION STATUS 0 Error from script
/opt/ccp4i/ccp4-6.1.1/ccp4i/scripts/import_scaled.script: can't read
"dataset_name": no such variable
#CCP4I TERMINATION TIME 02 Jul 2011 12
NMR people do collect NMR data beyond 40 deg C. Many membrane protein
structures are determined at 55-60 deg .C (the membrane proteins may not
be very large but
in micelles -combined weight is large). When the protein is stable and
the 1H-15N-HSQC (fingerprint region) does not change with temp (me
'NMR people' do use thermophilic proteins but I have never heard of anyone
boiling samples :)
By increasing temperature I meant going to around 37-40 deg C.
On 7/1/11 12:26 PM, "Jacob Keller" wrote:
>...they are stable enough that one can raise the temperature to increase the
>correlation tim
>...they are stable enough that one can raise the temperature to increase the
>correlation time.
I wonder whether NMR people have tried using hyperthermophile proteins
to be able to essentially boil the sample to decrease the rotational
correlation time constant? There is even an organism I read
Hello Kristof,
you could place dummy atoms with coot where you think the channel ends and
measure the distance between the dummy atoms - that's probably as accurate as
any automated tool because it takes you (human) experience into account.
Tim
On Fri, Jul 01, 2011 at 12:12:21PM +0200, Kristof V
Dear,
Does anybody know a conventional method/program for measuring the
dimensions (e.g. in Å), not the volume, of solvent channels in metal
organic frameworks (MOF's)..?
Thank you very much
Kristof
---
Kristof Van Hecke, PhD
Biomolecular Architecture
Cele
Thank you to all for the help. There’s obviously more than one way to skin a
cat! In the end pdbcur did the job for me. Great!
Dave
David Hargreaves
Associate Principal Scientist
_
AstraZeneca
DECS, CP&SS
Mereside, 50F
