[ccp4bb] How toto quickly evaluate the LLG(log-likelihood) value by given the structure factor information only

2011-04-30 Thread Hailiang Zhang
Hi, Is there any way to quickly evaluate the LLG(log-likelihood) value by given the structure factor information only (Fo, sigmaFo and Fc)? Thanks in advance for any information! Best regards, Hailiang

[ccp4bb] Met auxotroph and aa-mix

2011-04-30 Thread
Dear all, I want to make a Se-Met-labeled protein using the Met auxotroph strain B834. All the protocols I've found require addition of aa-mix. Has anyone expressed in this strain without the aa-mix? Any ideas and suggestions? Thanks in advance! Eric

Re: [ccp4bb] Error in LABIN

2011-04-30 Thread Jason C Porta
It was a permissions problem with the tmp directory. I thought I changed it, but alas it was set to root. Thanks for your help! Jason -Bruno Matias wrote: - To: Jason C Porta From: Bruno Matias Date: 04/30/2011 07:21AM Subject: Re: [ccp4bb] Error in LABIN Jason, I think you shoud