Dear ccp4bb,
I want to know regarding Wilson B value and mean B-value of protein after
refinement, In my case there is large difference in Wilson plot and
overall.Is it OK to have such value ie Wilson B-value 42 and mean B value is
23.3 ?
Also in the same structure I have observed that in a few res
As with all these things, YMMV.
In our experience, chaperone co-expression can help not at all, can produce
more folded protein or can produce more soluble protein, with co-purifying
chaperone. With GroEL/S at least, chaperone release is easy (add ATP) and
sometimes results in the liberation of
Dear Colleagues,
Based on the note below from Gerard Bricogne, I would like to further clarify
and highlight the intended purpose of the workshop for those of you who may be
interested in attending.
The planned discussions of the workshop will focus on the positive and useful
benefits of usin
You can change the target torsion angle for the double bond in the .cif file
that is created when your molecule is created.
--Subhangi
On Fri, Apr 22, 2011 at 3:11 PM, Jim Fairman wrote:
> I am attempting to create a monomer with a cis-double bond using Sketcher,
> but every time I tell sketch
I am attempting to create a monomer with a cis-double bond using Sketcher,
but every time I tell sketcher to create the library files it makes the
double bond a trans-double bond. Can anyone assist me with information on
how to force it to remain cis?
--
Jim Fairman, Ph D.
Post-Doctoral Fellow
N
Hi Michael,
It worked for me with one viral enzyme but did not work with 2 others. In the
successful case, I used Takara's chaperone plasmids to screen for the best
composition of chaperones. The GroEL-GroES improved the yield of the soluble
enzyme, but I got same activity (per 1L of media) as
First line up 10^9 poohbears...
Sent from my iPhone
On 22 Apr 2011, at 19:58, Jacob Keller wrote:
> I think soft crystallography uses fuzzy logic--it's a comforting
> alternative to the usual SAD and MAD.
>
I think soft crystallography uses fuzzy logic--it's a comforting
alternative to the usual SAD and MAD.
I think soft crystallography uses fuzzy logic.
JPK
On Fri, Apr 22, 2011 at 11:08 AM, Gerard Bricogne
wrote:
> Dear BC and colleagues,
>
> It is rather unclear from the title of the workshop whether it is the
> Crystallography that will be soft, or the X-rays.
>
>
> With best wishes,
>
>
Dear BC and colleagues,
It is rather unclear from the title of the workshop whether it is the
Crystallography that will be soft, or the X-rays.
With best wishes,
Gerard.
--
On Fri, Apr 22, 2011 at 11:56:22AM -0400, Bi-Cheng Wang wrote:
> We would like to call your atte
My collaborator was successful in purifying soluble rat phosphatidylinositol
transfer protein in E. coli when overexpressing GroEL/ES. They estimated ~50%
of the protein became soluble. We were able to solve the structure from
protein purified using this expression construct. We did not expe
We would like to call your attention to a workshop associated with the upcoming
2011 APS Users Meeting, which will be held on May 4, 2011 at the APS, Argonne
National Laboratory, USA.
The title of the workshop is “Soft X-ray Crystallography”. You may find the
workshop program at
http://20
Dear ccp4bb,
I am curious to hear of examples where the expression of well-behaved
protein was achieved by the coexpression of chaperones in E. coli. I
know the appropriate strains and vectors exist, but I can't remember
hearing of a successful case. I have heard anecdotally of several cases
wh
The CCP4 program UNIQUE does this.
http://www.ccp4.ac.uk/html/unique.html
"UNIQUE creates a unique list of reflections for a given unit cell with
a given symmetry up to a specified high resolution limit. The output
file can be used to complete a dataset (i.e. to give an MTZ file with
all allow
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