Hi,
Is there any ccp4 tool which can add a new reflection point (new hkl which
was not in the existing mtz file) to the present mtz file? Thanks!
Best Regards, Hailiang
Hi Maher
My version of Overlapmap reports the CC for main chain and for side
chain in the log-file (per residue and overall). Have you looked there?
-Bjørn
On 2011-04-21 14:25, Maher Alayyoubi wrote:
Hi Everybody, I posted a question earlier on the bulletin regarding
how to calculate the map
Hi Maher,
I posted a question earlier on the bulletin regarding
> how to calculate the map correlation coefficient using Overlapamp *or
> *
> *any other program*? My follow up question is, does anybody know how to
> calculate the map correlation coefficient for the main chain and side
> chain sepa
Hi Everybody, I posted a question earlier on the bulletin regarding
how to calculate the map correlation coefficient using Overlapamp or
any other program? My follow up question is, does anybody know how to
calculate the map correlation coefficient for the main chain and side
chain separately?
Th
Hi Amir, Ade, Mark and Tom,
Thanks a lot for your replies. Checking with BSA is a great idea and I
should've done it earlier. The BSA run for recent experiment is OK. I
cannot find a standard profile for my experiment one year ago. So I
guess that there was something wrong with the machine
Hi Zhen
-was concentration higher in old run? Might be monomer-dimer equilibrium,
for recent paper, see Benfield et al. (JBC, in press)
-we have a miniDAWN with a 'low-high-medium' setting in the back of the setting.
Inadvertently changing the setting scales the MW values up and down.
Have you run
Dear All,
I am having a consistency issue processing my SEC-MALS data. The sample is a
80KD protein which may form a dimer. The experiment I run a year ago shows a
major peak at 11.4 ml (GE S200 column), which is calcuated to have a MW of
144kD. There is a minor peak at 10.0 ml. In an effort to
Just a note to alert interested users of some (post-doctoral) research
associate opportunities in Australia:
http://employment.uow.edu.au/cgi-bin/job_details.cgi?id=23878
Dear All,
Thank you for the replies to my post. I received more than 14 response. The
summary is given below.
The suggestions were
- add another purification step.
* Ni-column
* remove His-tag
* Ni-column
* gel filtration
if you have done that: add another step to test for better crystals
