Re: [ccp4bb] OT: Covalent modification of Cys by reducing agents?

2011-04-16 Thread Nian Huang
Dear Horacio, How does TECEP compare to BME or DTT? People claim it is better, but I want some crystallographers' opinion? Nian On Sat, Apr 16, 2011 at 4:24 PM, Horacio Botti wrote: > Dear Mike > > BME readily autooxidizes (need for metal traces and dissolved O2). Is yours > a metalloprotein? I

Re: [ccp4bb] OT: Covalent modification of Cys by reducing agents?

2011-04-16 Thread Horacio Botti
Dear Mike BME readily autooxidizes (need for metal traces and dissolved O2). Is yours a metalloprotein? Is your buffer contaminated with metals? Those situations would make the case a bit different. If not, unless your BME stock is already oxidized, blocking of the accesible thiols with B

Re: [ccp4bb] Lattice sampling and resolution - a seeming paradox?

2011-04-16 Thread Joseph Cockburn
> Assume you have a one dimensional crystal with a 10 Angstrom repeat. > Someone has told you the value of the electron density at 10 equally > spaced points in this little unit cell, but you know nothing about the > value of the function between those points. I could spend all night > with a