Hi Anita,
We have had success setting up drops with TEV present. We simply added TEV at a
50:1 molar ratio and then set up the drops a couple of hours later. We went
from having twinned crystals at 3A to untwinned at 2A, the crystal form also
changed from orthorhombic to monoclinic, all in the
> this can screw up your statistics on twinning and this can be due to
> the NCS rotation
> axis (or will be based on what you say) parallel to a crystallographic
> symmetry axis.
> just check that. it should still refine though but this will have
> opposite effect to twinning,
> so it can be probl
Dear Friends,
I want to calculate RSR value of my protein and Ligand .
For this I have tried 2fofc.inp through CNS and MAPMAN RS fit
calculation but could not get satisfactory result.
I will be grateful if any body help me to solve this problem.I will
apppreciate your comment.
Hey Anita,
I would like to add to Artem's comment that you can also try and cleave the
protein at 30c for 2hr and then continue the cleavage overnight at 4c (you
should check and see that your protein can withstand 30c incubation for 2hr,
of course).
In regard to your non-diffracting crystals - you
Dear Kenneth,
IMO there is no resolution cut-off to decide to go from TLS to individual
anisotropic Bs. I use the number of reflections per atom. You are refining 9
parameters per atom so you need quite a lot. When I have>18 ref/atom I switch
to anisotropic. I try both isotropic and anisotropic
Respected Madam,
> Yes - that is true.
>
> Any crystal might be split, and give diffraction with overlapping
>lattices- ie show non-merohedral twinning. If you are lucky/careful you
>might only get a few spots which overlap after integration of one of
the >lattices- not enough to be detected as "t
Hi Kenneth,
I hope this will answer most of your questions:
http://www.phenix-online.org/newsletter/
see "TLS for dummies" and "On atomic displacement parameters..." articles.
Pavel.
On Thu, Apr 7, 2011 at 5:39 PM, Kenneth A. Satyshur wrote:
> peoples:
>
> I know that TLS is a group B factor
For starters, you could re-clone the protein with e.g. just a His tag or
move the tag to another end, or put some distance between the end of TEV
site and the protein; or perhaps use no tag at all -- or a different one?
Is it possible that the tag is messing you up - yes. Is it 'probable' - I
can'
Hi Crystallographers,
I am working of 23 Kda protein with a Nterminal His tag and a TEV cleavage
site.
I am getting crystals with the his tag and tev site intact, but they dont
diffract.
*Is it probable that they dont diffract because of the extra his tag and
the tev site?*
I am trying to get
peoples:
I know that TLS is a group B factor for regions of proteins that are moving the
same.
It is used in low res structures. But at what resolution does one begin
anisotropic, i.e
individual aniso for each atom, and leave TLS out. Or can one still use TLS to
first
compensate for large motio
Thanks Boaz.
I tried that too. But error was same.
What worked is removal of all models except 1 model and removal of all waters.
Thanks
Yogesha
From: Boaz Shaanan [bshaa...@exchange.bgu.ac.il]
Sent: Thursday, April 07, 2011 5:44 PM
To: Sollepura Yogesha
Sub
Hello Everyone,
I am trying to calculate shape complementarity of NMR structure (1U89) using
CCP4 Sc.
I get following error
$WARNING: NO CRYST CARDS READ$
$TEXT:Warning: $$ comment $$
WARNING: NO CRYST CARDS READ FROM XYZIN
$$
$TEXT:Warning: $$ comment $$
WARNING: NO SCALE CARD
> what does the twinning analysis by Phenix tell?
> if you haven't done it yet i would think you should run the processed
> data through phenix Xtriage and see
> what is the twinning analysis tell you.
> It also is a good program to solve your space group issues.
> I think you might need a mtz file
Dear colleagues,
This is just to remind you that the XXII Congress and General Assembly of
the IUCr (International Union of Crystallography) will be held in Madrid
(Spain) from 22-30 August 2011 and that your Spanish colleagues kindly
invite you to participate not only in the most important c
Yes - that is true.
Any crystal might be split, and give diffraction with overlapping
lattices- ie show non-merohedral twinning. If you are lucky/careful you
might only get a few spots which overlap after integration of one of the
lattices- not enough to be detected as "twinning" from the stat
Hi everyone:
I used parrot to do density modification, and it found ncs operators. The
operators found are:
Non-crystallographic operators:
-ncs-operator
-144.47,179.729,-144.321,18.7789,16.4179,19.809,16.4383,16.4507,19.809
-ncs-operator
-10.0955,179.846,169.903,27.2216,37.7585,38.88,27
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