Re: [ccp4bb] mono S vs. mini S vs. S-HyperD

2011-01-25 Thread Artem Evdokimov
Mini-S is not quite what you're looking for. It's 3uM particles and has a nice fat back pressure. Resource S-15 and Resource S-30 (with 15uM and 30uM average particle size) may be what you need. You may also want to take a look at monolithic columns because they develop a lot less back pressure th

Re: [ccp4bb] Full-screen stereo with Coot/PyMol/etc on 3D LCD displays?

2011-01-25 Thread Carlos R. Escalante
Dear Hudel, I have Zalman monitors M215W connected to an iMac and to a PowerMac and both pymol and coot work well. I also tested the monitors in my MacBook pro and they work fine. The MacBook pro has an NVIDIA GeForce GT 330M, the iMac has an ATI Radeon 5750 and the MacPro has ATI radeon 5770.

[ccp4bb] Postdoctoral Position Available - Structural & Cell Biology

2011-01-25 Thread Anand Kolatkar
Postdoctoral Position - Structural & Cell Biology The Scripps Research Institute in La Jolla, CA A Postdoctoral position is available to study the structure and function of protein-protein interactions involved in tumor growth. The position requires a recent Ph.D. in one of the natural sciences

Re: [ccp4bb] Off Topic: Fixed point sequence alignments

2011-01-25 Thread Martin Hallberg
Hi, "Indonesia" is great for this. http://xray.bmc.uu.se/dennis/ Cheers, Martin On Jan 25, 2011, at 10:50 PM, Katherine Sippel wrote: > Hi all, > > I was wondering if you know of a program that can do protein sequence > alignments around experimentally determined equivalent residues (i.e.

[ccp4bb] Off Topic: Fixed point sequence alignments

2011-01-25 Thread Katherine Sippel
Hi all, I was wondering if you know of a program that can do protein sequence alignments around experimentally determined equivalent residues (i.e. biochemists have determined via mutagenesis and kinetics that residues 3,6 and 9 of protein X are the equivalent of residues 7, 14, 21 in homologous p

Re: [ccp4bb] mono S vs. mini S vs. S-HyperD

2011-01-25 Thread Christian Roth
Hi Jonas, a Mini S has a even lower bead radius of 5 µm, if I remember correctly. Its available as prepacked column from GE. I have a 1 ml column which is quite expensive. i guess you won't spent such an amount of money. It is also more for analytical purposes and not preparative scale. Never h

[ccp4bb] mono S vs. mini S vs. S-HyperD

2011-01-25 Thread Jonas Boehringer
Dear All, I am currently looking into various cation exchange resins for the separation of mono-, di-, tri-, tetra-,... ubiquitin. So far I have been using a small Mono S column but would ideally like to both scale up and increase resolution. Before I started searching for this online I have nev

Re: [ccp4bb] Se-Met Protein Purification

2011-01-25 Thread tommi kajander
I think the likely reason here are the points where you did the mutations having adverse effects - how do the mutants behave without labeling? this would be of course an important control... -Tommi On 25.1.2011, at 12.52, Paula Salgado wrote: Dear Abhilash You could try an alternative la

Re: [ccp4bb] Se-Met Protein Purification

2011-01-25 Thread Paula Salgado
Dear Abhilash You could try an alternative labelling option: if your protein has cysteines, try labelling those with selenium. We succeeded in doing this using non-auxotrophic strains, meaning you deviate less from your native expression protocol and are therefore more likely to obtain well folded

[ccp4bb] Se-Met Protein Purification

2011-01-25 Thread Abhilash Padavannil
Dear All, I am working on structure determination of a bacterial protein. The protein was expressed as a GST fusion protein in E. coli. I got crystals of the native protein. I am trying to label my protein with Se-Met. The purification protocol involves GST affinity purification, cleavage of the

Re: [ccp4bb] Full-screen stereo with Coot/PyMol/etc on 3D LCD displays?

2011-01-25 Thread Chris Richardson
On 25 Jan 2011, at 08:35, Harry M. Greenblatt wrote: > Has someone received an official answer from NVIDIA that they do *not* work? I raised this issue here back in November. Someone, whose email I cannot now find, replied off-list to say that they had been told by nVidia that 3D Vision did no

Re: [ccp4bb] Full-screen stereo with Coot/PyMol/etc on 3D LCD displays?

2011-01-25 Thread Harry M. Greenblatt
BS"D According to the NVIDIA website, the Mac versions of the Quadro FX 4800 and the Quadro 4000 have 3 pin stereo ports, and there is nothing that I could find that says that the MAC drivers for these cards don't support quad-buffered stereo using the new LCD screens and NVIDIA glasses