Re: [ccp4bb] PDB deposition

2011-01-03 Thread Wangsaner
Dear all, I have just received response from the PDBj Annotator through CCP4BB My PDBs will be processed within two weeks As some of you mentioned, the reasons of delaying processing time is due to holiday season and the extremely large amount of depositions recently Again, thank you

Re: [ccp4bb] PDB deposition

2011-01-03 Thread Wangsaner
Dear all, Thank to your replies, I am so released now! Thank you a lot for all responses. Best regards.

[ccp4bb] PDB deposition

2011-01-03 Thread Wangsaner
Dear all, Last year, when I deposited PDBs using ADIT, immediately I got an automatic confirmation email. And after 1 day, I got the responses from the PDBj Annotator. And within 3 days, all the edition and validation of my structures were finished with the help of member from the wwPDB. I g

Re: [ccp4bb] help with kinase purification

2011-01-03 Thread Michael Thompson
Hi Neeraj, A few points to add to Artem's excellent advice: If you would like to purify an untagged construct of your kinase, you could try an ATP affinity column. The caveats to this are 1.) the ATP resins can be expensive, and therefore it may be impractical to make a column with a high bind

[ccp4bb] Postdoctoral position in Brisbane Australia

2011-01-03 Thread Bostjan Kobe
Sorry for posting this again, but the web address of the full ad (see below) has changed. The deadline is 10 January. Happy New Year! POSTDOCTORAL POSITION IN MACROMOLECULAR CRYSTALLOGRAPHY, UNIVERSITY OF QUEENSLAND, BRISBANE, AUSTRALIA Applications are invited for a post-doctoral position in m

[ccp4bb] Only four weeks left to apply for RapiData 2011, our course on Data Collection and Structure Solving at the NSLS.

2011-01-03 Thread Robert Sweet
We are offering RapiData 2011, the thirteenth offering of our popular course: Rapid Data Collection and Structure Solving at the NSLS: A Practical Course in Macromolecular X-Ray Diffraction Measurement The course will be held 3-8 April 2011. Students could be at any level from adv

Re: [ccp4bb] SDS and IMAC

2011-01-03 Thread Phoebe Rice
Especially if you're dealing with lysate, I suspect the best way to do it is with magnetic Ni beads that you lift up and out of the gunk, to help avoid false positives from aggregating stuff that SDS/urea/guan would all "elute". But why do you want X to remain on the column/beads? Removing Y bu

Re: [ccp4bb] Anyway to change the toolbar position in coot?

2011-01-03 Thread Nian Huang
That's great. The 3D screen we got is only 23 inch. A full screen GUI like in the game will certainly be helpful. Nian Huang, Ph.D. Dept of Biochemistry UT Southwestern Medical Center Dallas, TX 75390 On Fri, Dec 31, 2010 at 5:00 PM, Nian Huang wrote: > Dear all, > The python version coot has tw

[ccp4bb] Post-Doctoral Position Available

2011-01-03 Thread Jean Cavarelli
POST-DOCTORAL POSITION DATE OF AVAILABILITY: starting as soon as possible, for 2 years. TITLE: structural biology of complexes involved in tumor reversion. POSITION: the position is opened in the department of Integrated Structural biology, IGBMC, located in Strasbourg-Illkirch (France) in t