Re: [ccp4bb] Resolution and distance accuracies

2010-12-24 Thread Pavel Afonine
Here is another set of relavant papers and instructive pictures: 1) CCP4 Newsletter http://www.ccp4.ac.uk/newsletters/newsletter42/content.html On the Fourier series truncation peaks at subatomic resolution Anne Bochow, Alexandre Urzhumtsev 2) Some Facts About Maps (pages 30, 32, 33): http://www.

Re: [ccp4bb] FW: [ccp4bb] Resolution and distance accuracies

2010-12-24 Thread Bernhard Rupp (Hofkristallrat a.D.)
> I find the "super-resolution" claims in this paper a bit of a conjuring trick. I think it is understood that information cannot come from nothing. You cannot cheat in basic physics. Interestingly, I had the same discussion with bioinformatics colleagues a short time ago. The problem is the same

Re: [ccp4bb] Resolution and distance accuracies

2010-12-24 Thread Bernhard Rupp (Hofkristallrat a.D.)
In reference to the low and high B atoms in a map and the ripples, one can actually calculate something of educational value using my web applets (with all the caveats imposed by simplicity of the 1-d case). Here is how: Go to http://www.ruppweb.org/new_comp/structure_factors.htm and set the B-valu

Re: [ccp4bb] SDS and IMAC

2010-12-24 Thread Artem Evdokimov
Use Urea - it does not interfere with gels etc. Additionally, you should consider covalent immobilization of protein X - using activated resins. Amine and carboxylic acid immobilization is common, however my all-time favorite is iodoacetamide-activated resin reacting with SH on the protein (if you

Re: [ccp4bb] Resolution and distance accuracies

2010-12-24 Thread Jacob Keller
To get an idea about this signal:noise issue, I wonder what the B factor of bulk solvent would be, if it is possible to think in such terms, i.e., fill the bulk solvent with dummy waters and let the B's do what they will. I wonder whether anyone has tried explicitly modelling the whole unit cell th

Re: [ccp4bb] Resolution and distance accuracies

2010-12-24 Thread Ian Tickle
I have a program which computes the atomic electron density profile (attached) as you would see it in a map, using accurate scattering factors and taking the resolution limit into account. I wouldn't call the profile for a C atom with B=100 at 2.5 Ang resolution 'flat', maybe 'flatter'. 'Flat' wo

Re: [ccp4bb] sudhir.1...@gmail.com is inviting you to freecharge.in

2010-12-24 Thread faisal tarique
is these site is meant for this purpose ? On 12/24/10, Bosch, Juergen wrote: > I only drink Lavazza sorry. Donations can be sent to below address. Thank > you ! > > Jürgen > - > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns

Re: [ccp4bb] sudhir.1...@gmail.com is inviting you to freecharge.in

2010-12-24 Thread Bosch, Juergen
I only drink Lavazza sorry. Donations can be sent to below address. Thank you ! Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-