[ccp4bb] superposing (hkl) indexes on diffraction image

Dear all, I would like to make a picture of diffraction photograph with (hkl) indexes. I found it in Fig. 1 in the paper: Acta Cryst. (2009). D65, 553-559 http://dx.doi.org/10.1107/S0907444909010725 Direct link to the figure: http://journals.iucr.org/d/issues/2009/06/00/dz5158/dz5158fig1.html Ca

Re: [ccp4bb] How to use XDS programme to process data collected at Q315r detector

Donghui, there are several ways to get ORGX ORGY parameters. As Juergen suggested, you could use the numbers from the header or Mosflm output. More information on the excellent XDS Wiki: http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Obtaining_ORGX_ORGY Another option would be to r

Re: [ccp4bb] How to use XDS programme to process data collected at Q315r detector

Look into the header of your image file via more or run mosflm on one image and look at the output in the terminal window. You'll get the pixelsize and you'll need to convert the beamcenter into pixels from mosflm. Keep in mind that beam X = orgy in xds and beam y is orgx Plus the usual stuff la

[ccp4bb] How to use XDS programme to process data collected at Q315r detector

Dear all, Recently I collected several data sets at 13B1 Taiwan beamline with Q315r detector. It's no problem to index these datasets using mosflm, but Rms residual and weighted residual is high. Here I want to try XDS to play my data. I downloaded a template example as below. !**

Re: [ccp4bb] brute force MR

Try this, assuming you have good data. Use one molecule you got to do refinement (rigid body or restraint refinement with tight restraint) and phase the map. Then do a phased map molecular replacement. You might want only use the core of your protein to do the molecular replacement search to avoid

Re: [ccp4bb] brute force MR

Just a thought: shave off parts of your model1, which you think might cause a clash perhaps, essentially keeping a core in place, then rerun molrep using model1 as fixed and search for a second. If you are successful, then SSM superimpose your complete model onto both and see what you have to fi

[ccp4bb] brute force MR

Hi there: The situation: We are facing difficult molecular replacement: we believe we have two molecules in the ASU, but phaser/molrep find only one. Using the electron density calculated using this single molecule, we have manually placed the 2nd molecule, albeit not good enough for rigid bo

Re: [ccp4bb] salt or protein crystals?

Definitely small molecule crystals. You might want to push the detector closer and use better cryo solution for further confirmation. Nian On Tue, Dec 7, 2010 at 8:14 AM, xiuwen zhang wrote: > Dear Colleagues, > >     Currently we got several very tiny crystals. After exposuring a cluster > of c

[ccp4bb] BM14 Beamtime Call for Feb-March 2011.

Dear colleagues, Since January 2010, the BM14 MAD MX beamline at the ESRF (Grenoble, France) is providing beamtime for both European and Indian MX communities after a smooth transition from the UK MRC-EMBL tandem to the actual EMBL-ESRF-India consortium. The beamline is fully opened now fo

Re: [ccp4bb] salt or protein crystals?

I agree - looks like small molecular diffraction. Try increasing delta-phi to catch more of the lattice to confirm - I often do a 5º image or two with the detector pushed as close as possible to check for salt diffraction when screening. The lack of low res (~15-20Å) spots around the beamstop is

Re: [ccp4bb] pH dependent conformational change

I would like to point out that HSQC could still be applied even in such a large protein. TROSY-HSQC has been successful in improving peaks in spectra of large protein. Typically the sample would need to be deuterated to see the full effect of TROSY, but even a partial deuteration can improve sig

Re: [ccp4bb] salt or protein crystals?

Looks like diffraction off of tiny small-molecule crystals to me. Artem On Tue, Dec 7, 2010 at 8:14 AM, xiuwen zhang wrote: > Dear Colleagues, > > Currently we got several very tiny crystals. After exposuring a cluster > of crystals one hour in home source, we could find some weak diffracti

[ccp4bb] MX Post-Doc postion at Diamond

Dear all, we have a vacancy for a postdoc within the MX group at Diamond. Full details can be found at http://www.diamond.ac.uk/Home/Jobs/Current/DIA0584-TH.html. Interested candidates can contact me directly by email for further details on research project associated with the post Martin

Re: [ccp4bb] pH dependent conformational change

Hi Intrinsic fluorescence may be used to monitor such change qualitatively. Another option is red-edge excitation flourescence technique may work if there are suitable fluorophores (W or F) on the each of the two domains changing solvent accessibility upon rigid body motion. Amit. On Mon, Dec