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Jürgen
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Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-
Hi all..,
How to get a PDB of small molecule which is not available in data base..??
is there any server that provides a area where in we can do a chemdraw and get
the 3D picture of it and also PDB format
plz help.
Thank you,
Regards,
Hussey
hear-hear
wise words.
may I add that if you are buying a DLS anyway, maybe consider getting
a model which can also be connected online to an FPLC to do Static LS
measurements,
... after all a DLS signal is basically an autocorrelation of the
static signal over time ... which will tell you
Hi Shukuri,
If you're on a tight budget and you only want to use DLS to verify sample
homogeneity I would save my money or use it for something else (a
crystallization robot, for example). You definitely do NOT need DLS to solve
crystal structures, including those of membrane proteins. Many mon
I second the question of need: a decent PCR machine capable of
'thermofluor'-like experiments should cost around $34-$38K, which leaves
about the same amount of $$ for a decent purification machine. One of the
questions you have to figure out is: are you setting up a high-throughput
lab or a regula
instead of DLS you could also look into buying a fancy PCR machine ~30K where
you can perform
a) regular PCR
b) quantitative PCR
c) thermal stability tests for your protein
Ericsson et al. Thermofluor-based high-throughput stability optimization of
proteins for structural studies. Anal Biochem (
On Saturday, October 30, 2010, Boaz Shaanan wrote:
> Hi,
>
> I'm not sure why you want to carry the free R reflections from the small cell
> to the new cell. If it's the model bias vis-a vis the reflections
> participating the refinement that you want to get rid of you can take another
> route,
What is your question?
Do you mean you are new to DLS or new to crystallography?
Is it wise to spend your start-up funds in buying something (you know little
about) for which you have to depend on the blog for major decisions rather than
investing in something you know well and you can use immedi
Hi,
I'm not sure why you want to carry the free R reflections from the small cell
to the new cell. If it's the model bias vis-a vis the reflections participating
the refinement that you want to get rid of you can take another route, I think.
1) Select R-free set for the new cell (paying attenti
Hi Ed,
in the new cell (long a axis), the reflections H K L are related by
H=2*h K=k L=l to those of the old (short a) cell. I would expect that
the R-factor of those H K L reflections with even H from the new crystal
form is low (at least at low resolution) against the h k l reflections
of t
Peter
Yes, by "2Fo-Fc type maps " I was including the weighted maps you
mention (which should be better).
The main issue is whether 3mF(obs)-2DF(calc) and higher coefficients
would be better for the twinned reflections
Regards
Colin
From: CCP4 bulletin
Fig from John (I have a copy of his book in the office and will see it only
tomorrow) due to better quality
makes it clear (at least for me) that we see the similar effect shown by David
Goldstone.
Dr Felix Frolow
Professor of Structural Biology and Biotechnology
Department of Molecular Micro
I have seen such features myself from time to time on diffraction pictures
recorded on X-ray films in the far past and was explaining them by the presence
of electron diffraction as they resemble electron diffraction patterns.
Source of focused electron beam during X-ray diffraction experiment is
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