[ccp4bb] [OFF-TOPIC] Science is Vital.

Hi all. Apologies for the off topic, largely UK-centric posting. In a period of fiscal uncertainty in which most governments (USA, India, China, France, Germany) are actively increasing their investment in R&D, the UK government is seriously considering cuts of up to 25%, if press/analyst reports

Re: [ccp4bb] Differences between refmac_5.5 and refmac_5.2

Hi the reason could be that refmac converts all aniso B to iso B when you do overall B value refinement. Without data it would be difficult to test and give definite answer. regards Garib On 9 Oct 2010, at 00:15, Hailiang Zhang wrote: > Hi, > > I found there are many changes between refmac_

[ccp4bb] Differences between refmac_5.5 and refmac_5.2

Hi, I found there are many changes between refmac_5.5 and refmac_5.2. For example, the key word "REFI BREF OVER" will result in totally different results under these 2 versions. Based on my input PDB with anisotropic B pre-refined, refmac_5.5 gave a much higher R/Rfree than refmac_5.2. Can somebod

Re: [ccp4bb] Removing a tight binding ligand

I would like to thank all of you for your replies. I very much appreciate your time and I've got some great starting points to try out. I will put together a recap of the off- and on-board comments in the next day or so for archive posterity. For those looking for more details... The protein

[ccp4bb] unknown ligand density

Hello all, I have a structure of my protein at 1.3 Ang. There is a clear density in the binding site but I can't seem to figure out what the ligand is. The crystalization conditions contain PEG, sulfate, TMAO and acetate buffer. The protein was purified by a Ni-NTA column and went into phosphate

Re: [ccp4bb] Removing a tight binding ligand

It is just like the regular dialysis but the deserved buffer contains charcol powder. Meng-Chiao Joseph Ho > How do you do this ? > I have not heard of this, but I also never had to deal with getting rid of > a ligand. > However I would be interested to learn more about this method. > > Thanks, >

Re: [ccp4bb] Removing a tight binding ligand

Hi, It could help if you said what your ligand is. Nadir Pr. Nadir T. Mrabet Structural& Molecular Biochemistry Nutrigenex - INSERM U-954 Nancy University, School of Medicine 9, Avenue de la Foret de Haye, BP 184 54505 Vandoeuvre-les-Nancy Cedex France Phone: +33 (0)3.83.68.32.73 Fax: +33 (0

Re: [ccp4bb] Removing a tight binding ligand

Hi Katherine, We had a case where we used saturating amounts of NaCl and precipitated the protein to get rid of a very tight binding ligand (Structure, 11, 677-690, 2003; look at the "preparation of the apoenzyme" section). Regards, Mathews -Original Message- From: CCP4 bulletin boar

Re: [ccp4bb] Removing a tight binding ligand

We often dialysis protein against charcoal to remove small molecules that tightly bind to protein. Meng-Chiao Joseph Ho, PhD Department of Biochemistry Albert Einstein College of Medicine > Hi all, > > I am working with a substrate binding protein. The protein > scavenges its endogenous ligand out

[ccp4bb] Call for applications to the Membrane Protein Lab at Diamond

Call for applications to the Membrane Protein Lab at Diamond Dear Crystallographers, The Membrane Protein Laboratory (MPL) at Diamond Light Source in Oxfordshire (UK) is pleased to announce that is now open our call for proposals. The MPL is a joint venture between the Diamond Ligh