[ccp4bb] Which is the better way to combine the dataset from different crystals?

Hi all, I have a question on how to combine two dataset from different crystal of the same protein. The first way I could think of is that, 1) Merge two dataset seperately; 2) Scale them to see whether they are consistent with each other enough as indicated by the R-factor; 3) If the R is low eno

Re: [ccp4bb] Density changes from Positive to negative after ligand addition and refinement

Thanks James and all of you, The occupancy had to be lowered to 0.25 to get the B-factor similar to the coordinating atoms. But now I can see a good positive density. Ivan On Tue, Jun 29, 2010 at 7:49 PM, James Holton wrote: > > This kind of thing happens when the metal is not at 100% occupancy

[ccp4bb] Viscotoxin A1 S-SAD example data

Dear BBs, in response to multiple requests, the Viscotoxin A1 S-SAD sample data (from unmerged hkl data forward) used in BMC Chapters 8,10,12 are now available, courtesy of Aritra Pal and George Sheldrick. Use pull down 'My BMC Book' on my website, select Example Data. Best, BR ---

[ccp4bb] Project Leader Electron Microscopy - position available at PDBe

The Protein Data Bank in Europe (PDBe; pdbe.org) has a vacancy for the Project Leader Electron Microscopy position. For more information: http://www.embl.de/aboutus/jobs/jobs_embl_ebi_hinxton/2010/w_10_059_ebi/index.html PDBe is the founder of the worldwide archive of 3D cryo-EM data, the Ele

Re: [ccp4bb] UV microscope

If you check http://formulatrix.com/product_crystallizationimaging_muvis.html, you will find a stand-alone UV microscope. I have enjoyed using the RockImager UV microscope, which is an integrated hotel/imaging station. The MUVIS is a bench-top standalone imaging system that should supply what you n

[ccp4bb] ccp4MG quits - mac

Greetings. I have been using CCP4MG to create images on my Mac OS 10.6.4 and have been having problems with it unexpectedly quitting. It appears to be linked to either large changes (such as having a surface displayed and then changing the properties of that surface) or displaying things like

[ccp4bb] OFF TOPIC: Akta Prime + Hg lamp replacement

Dear all, Sorry for the irrelevant topic. Does any one know where to buy the uV lamp for an Akta Prime+? This is sold by GE but it is hugely expensive and is setting me off £579. Any way to get a more resonably priced replacement? Hg lamp assembly for Akta Prime: 18-1128-22 Thanks a lot.

Re: [ccp4bb] vacuum adapter for Dry shipper Cryo Dewar

When I researched this a number of years back, both Taylor-Wharton and Cole-Parmer techs said nearly all dewar failures were in the epoxy in the neck of the dewar. This could not be remedied through pumping down the vacuum jacket as the seal was breached. They did give some advice to elongate

[ccp4bb] Welcome to the new PDBe website (pdbe.org)!

PDBe launches its redesigned website (pdbe.org) --- The Protein Data Bank in Europe (PDBe; http://pdbe.org) has launched its redesigned website with lots of new features, enhancements and improvements. This so-called "Midsummer Make-over" is the first

Re: [ccp4bb] fsck problem.

Step 1: got www.ixquick.com and paste in the error message (fsck.ext2: Is a directory while trying to open /), read the first link of the search results. Step 2: Enter the root password at the (possibly not well visible) prompt and run fsck on your partitions. Step 3: mount

Re: [ccp4bb] Wilson B and Mean B factors

Dear Murugan, at higher resolution, the Wilson plot captures mainly the contribution of atoms with lower B-factors which leads to a systematic underestimation of the true B-factor distribution. Accordingly, the average B-factor of refined structures tend to be higher then the Wilson B-factor