I know of one bad experience with a common refrigerator as it pertains to the
freezer. The refrigerator was great for the 4 deg crystallization trials, but
the freezer had a "frost-free" cycle which was destroying protein and
chemicals. The user was keeping lots of their sensitive items in the
Hi -
did you express your secreted mammalian protein in the E.coli
periplasm? If so, and if the oligomerization state is well defined, eg
its a 4mer, 5mer or whatever, and not a mixed population, I would be
quite confident that this is the 'true' native state. But, I would
only be sure in
Any protein will absorb at 230, and most UV detectors these days can
reach that, if you want to follow that with UV.
Together with SDS_PAGE of course, this should be good enough to go
ahead.
A.
On May 28, 2010, at 18:03, Sollepura Yogesha wrote:
Dear All,
I have expressed 30-40 aa region m
In fact, you can put plates in refrigerator. It is cheap and simple. Maybe the
temperature is not stable, but don't warry, acturaly, the dynamic temperature
is benifit to grow crystals. You can look for relational papers.
Of course, you can buy a incubator, which can control temperature from 4-6
Hi ccp4ers,
I found a problem when I used SHARP 2.6.0 Sushi 3.8.0 to search new heavy
atom sites from 2 identified sites from SnB. Sharp can output new identified
site lists however with all new identified sites set distance at zero with
the 2 sites and strangely the 2 sites all output as G0 site.
