Dear Dirk
> For REFMAC, I
> haven't seen anything similar, yet.
The external restraints in Refmac should allow you to do this.
http://www.ysbl.york.ac.uk/~garib/refmac/data/refmac_news.html
Best
Roberto
>
On Wed, Mar 31, 2010 at 5:57 PM, Muhammed bashir Khan <
muhammad.bashir.k...@univie.ac.at> wrote:
> I am working with protein which are supposed to be bound with Magnesium
> and Cobalt metals.I have now its crystals which are diffracting quite
> nicely.I want to know the Magnesium and Cobalt bindi
Dear all;
I am working with protein which are supposed to be bound with Magnesium
and Cobalt metals.I have now its crystals which are diffracting quite
nicely.I want to know the Magnesium and Cobalt binding residues in the
target protein. When I add magnesium or Cobalt salts to protein solution
Hi All
Seems Roberts suggestion has worked.
Thank you Robert
Best Gina
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Clayton, Gina Martyn
Sent: Wednesday, March 31, 2010 4:34 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] B factor Coloring
Hi Robert
That seems like a great suggestion! I will give it a shot and let CCP4BB
know if it works
Thanks
Gina
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Robert Campbell
Sent: Wednesday, March 31, 2010 4:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Su
Hi Ron
Mmmm...
not really but thanks for the info:)
I have my Bfactor column assigned in percent conservation so the B value
ranges from
say 30% to 100%. I want to colour my molecule accordingly. Pymol allows
B factor
colouring but seems only to allow 3 colour choices. It used to allow 4
colou
Hi Gina,
On Wed, 31 Mar 2010 15:00:34 -0400 "Clayton, Gina Martyn"
wrote:
> I am trying to colour a structure by B factor in Pymol. More
> specifically I am colouring conserved residues (value in b factor). I
> want to use 4 colours - say white, yellow, orange, red. However it
> seems that Pymo
Hi,
I'm aware of two ways to do this:
by writing the colour as a word, e.g:
color white, (mypdbfile//A/*/*)
which will colour a whole chain.
Or by using rgb colours, e.g:
set_color newcolor0 = [1,1,1]; color newcolor0, (mypdbfile//A/2/*)
which will colour individual residues (residue 2 in this
Dear Everyone
Slightly off topic...
I am trying to colour a structure by B factor in Pymol. More
specifically I am colouring conserved residues (value in b factor). I
want to use 4 colours - say white, yellow, orange, red. However it
seems that Pymol now only allows 3 colours to be used - I t
Not strange at all:
Reading from:
http://www.chem.gla.ac.uk/research/groups/protein/mirror/stura/cryst/add.html
"Zinc acetate or sulfate 0.2-5mM It inevitably reduces protein solubility.
It can act as an inhibitor. Essential for activity of various enzymes"
Zinc reduces protein solubility an
On Wed, Mar 31, 2010 at 8:47 AM, Dirk Kostrewa
wrote:
> this is very interesting! From the list of changes, it appears that in
> version 1.6.1, you use a similar idea to implement hydrogen bonds via DSSP
> during refinement that I used to stabilize the 4.3 A refinement of Pol II in
> complex with
Hi Dirk,
this is very interesting! From the list of changes, it appears that in
version 1.6.1, you use a similar idea to implement hydrogen bonds via
DSSP during refinement that I used to stabilize the 4.3 A refinement
of Pol II in complex with TFIIB [1]:
(...)
Could you please give more deta
hello everyone， recently i purify a protein conteining zinc binding
domain,and i want to determine its structure.i get the crystal,but poor
diffraction.so i try to adding zinc into the protein to optimize the
crystal,but the protein precipitate immidiately even the znic is 1
mM.BTW,we use the pro
hello everyone, recently i purify a protein conteining zinc binding domain,and
i want to determine its structure.i get the crystal,but poor diffraction.so i
try to adding zinc into the protein to optimize the crystal,but the protein
precipitate immidiately even the znic is 1 mM.BTW,we use the
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Dear Paul,
this is very interesting! From the list of changes, it appears that in
version 1.6.1, you use a similar idea to implement hydrogen bonds via
DSSP during refinement that I used to stabilize the 4.3 A refinement of
Pol II in complex with TFIIB [1]:
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