Re: [ccp4bb] AW: [ccp4bb] off-topic: crystal optimization without buffer

Hi Katja, If your protein preparation did have salt, you could try lowering that salt (if your protein tolerates) and grid search with varying (increasing) PEG concentrations. Seeding and Additives should really help. the effect/outcome is difficult to predict but these two techniques are helpful i

Re: [ccp4bb] Bad geometry for alt. conformation refined in Refmac5

I think I've finally squashed this particular bug which had been annoying me for quite some time! It appears that Intel Fortran v11.0 has an optimisation bug in s/r CHK_ALT (i.e. checking alternate atom codes), & possibly also 10.x - I don't have those to test - though v9.1 & possibly earlier seem

Re: [ccp4bb] decrease of background with distance?

John Another point which might resolve some of the confusion regarding detector distances (or add to the confusion) " that moving the detector back was not the setting required but a small collimator (0.2mm) and slitting down the divergence to control the spot size versus the broader halo of acou