try codon optimisation
use Rosetta 2 strains
fuse to MBP
I expressed a yeast protein of ~100 KDa, expression was low until I fused it
to MBp (resulting protein ~ 150 KDa)
Dear All,
In refining my structure (two molecules in au) that was solved with molecular
replacement, refinement starting with restrained lowered the R-free to about
0.35 while with NCS increased the R-free to 0.55. Does it imply that two
molecules are quite different or something wrong with th
Hi, I just installed the 64-bit version of Ubuntu 9.04 Desktop, and then
I installed CCP4 6.1.1 using the standard package and installation
script. I can run ccp4i successfully. However, when I try to run Coot,
I get a bunch of errors. I think the problem is that Coot was compiled
with 32-bi
In short, yes you can. Although it is often avoided due to the limitations
that high salt samples may have, you still have a good chance to
crystallize the protein in the original high salt buffer. In my case a
small alcohol was the best precipitating agent. As with most proteins, try
it, try it, t
Posted on behalf of Wolfram Saenger:
X-ray equipment to give away
Since I am close to retirement, the equipment I have will be given away
at no cost except for transport. The equipment are two mar research
image plates, 345 and 250 mm diameter, the first running under PC, the
secondhas an interfa
Thanks to all who answered!
Removing "RUN 1 reference" did the trick
Another idea was to simply:
EXCLUDE batch 551 to 650
but then, smoothed scaling is not possible (to wide a gap )
Best regards,
Miguel
2009/7/3 Miguel Ortiz Lombardia >:
Dear all,
I'm trying to scale a dataset where 100
Dear all,
I'm trying to scale a dataset where 100 frames in the middle (25
degrees) are quite bad so I would like to exclude them. Because the
angular range is too wide I can only do that by creating two runs. So
far, so good. The problem is that even if my input contains these five
lines
Replies to kamzh...@riken.jp
Postdoctoral Fellow Position in Computational Structural Biology at the
Zhang Initiative Research Unit, Advanced Science Institute, RIKEN, Japan
We are seeking a highly motivated and experienced computational
structural biologist to join the Zhang Initiative Researc
Call for applications to the Membrane Protein Laboratory at Diamond
Applications are invited for proposals to use the Membrane Protein
Laboratory (MPL) at Diamond Light Source Ltd.
Closing date for applications: 28th July, 2009.
MPL website: http://www.diamond.ac.uk/Home/MPL.html
The MPL
Jacob
Before you spend you holiday weekend "dephaging" the lab and picking up on a
comment from Artem and yourself, your protein could indeed be toxic to the
cells. You do state in your email that the your are working with new
constructs. What happens with your old constructs ? I've been unfortunat
Jacob,
You should be able to get rid of the problem within a few days as long as
everyone in the lab is willing to cooperate.
Autoclaving works (it helps to use longer autoclave cycles - 40 minutes
instead of the regular 20).
If you confirm that phage is your issue - walk through the lab and car
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