Gerard DVD Kleywegt wrote:
Congratulations, James. But surely you could have found a better
example of medical imaging? For instance, here:
http://www.ibeatyou.com/competition/867945/craziest-x-ray/entry/be24b3/coke-bottle
--dvd
Unfortunately, (unlike the infamous coke bottle image),
Most of the poorly cleavable fusion proteins (usually MBP-TEV) that
I've seen turned out to be solubly aggregated.
ho
UC Berkeley
--
Date:Fri, 27 Feb 2009 07:23:43 -0500
From:Stephen Weeks
Subject: Re: Off topic: Mammalian gene expression in E. coli
Just i
Hello,
The short answer is 'yes'. If you can use both methods :) The issue with
limited proteolysis lies in the questionable state of the full-length
protein - if the stuff is nasty and misfolded, then fagments generated by
proteolytic digest aren't going to be meaningful. On the other hand if you
I even made the cover! And I hope that (after looking at the cover) all you
BBers will forgive me just this once as this was clearly a once-in-a-lifetime
opportunity. Or, at least, I HOPE only once.
Congratulations, James. But surely you could have found a better example of
medical imaging?
As much as I hate to see the CCP4BB used for shameless self-promotion of
articles, I did just publish a "Beginner's Guide to Radiation Damage":
http://journals.iucr.org/s/issues/2009/02/00/issconts.html
My answers to Paul and Michael's posts would be cut-and-pasted from it,
so I think it approp
Michael:
I attended the CCP4 study weekend in January and there were many discussions
about radiation damage. In your case I wonder if recollecting the data with
a lower over all dosage might get rid of the problem. Either collect with
an attenuated beam or collect the data twice as fast. Someo
Dear Sergei,
We have recently had a Microstar installed from Bruker. It had some serious
teething problems and it took close to a year from the point of
installation in our building until it could be used for any data
collection. Many parts of the original machine delivered had to be replaced
ar...@xtals.org wrote:
Hi,
In a case like the one Raji outlined below - after all the attempts - I
would have most cerainly switched to insect cells as the next step :)
If you suspect that protein of interest has large disordered regions,
expression in a higher order system by itself may not be
Dear All,
following a good discussion recently on the crystallization
imaging systems, I wanted to start a discussion with respect
to choosing a new in-house X-ray source (rotating anode).
Bruker is there with Microstar-H and Microstar Ultra.
Rigaku has 007HF and FR-E+.
I wondered if people who
Hi,
In a case like the one Raji outlined below - after all the attempts - I
would have most cerainly switched to insect cells as the next step :)
If you suspect that protein of interest has large disordered regions,
expression in a higher order system by itself may not be enough (still
good chanc
I can't help on software but this would explain Gordon Brown's
presence:
http://www.google.com/hostednews/ukpress/article/ALeqM5jQKS8GcXtpz_FvAK08ybc5QRfRBQ
Isn't google amazing?
Frances
=
Bern
It would be possible to model all of that with SHELXL, but it is a complex
problem and would need time and patience with any program. One advantage
of SHELXL would be the flexible refinement of occupancies for the
different conformations.
George
Prof. George M. Sheldrick FRS
Dept. Structural
Dear experts,
here is a Refmac question (or at least a refinement question):
the case: data set was collected at the synchrotron with high intensity or
better high total dose. Data set scales to 1.2A resolution.
Refinement with refmac5_0066, using hydrogens, anisotropic B-factors, automatic
wei
Just in case anyone else encounters the same problem, like Artem I
have had a few SUMO fusion constructs that have stubbornly refused to
cleave even with a 1mg of enzyme (Ratio ~ 1:100) at 37c in the presence
of low concentrations of chaotrope. In all cases the problem was solved
by inserting
Partha Chakrabarti wrote:
Hi,
I am running a 64 bit Fedora 10 machine and having problems with coot,
if i say
ldd coot-real
linux-gate.so.1 => (0x5000)
libguilegtk-2.0.so.0 => not found
libgthread-2.0.so.0 => /lib/libgthread-2.0.so.0 (0x00df8000)
librt.so.1 => /lib/librt.so.
Coot sets up the paths before it starts. That's why you don't call
coot-real but the script coot which modifies LD_LIBRARY_PATH and some
other environment variables before actually calling the binary. Therefore
applying ldd to coot-real is not telling you much unless you set
LD_LIBRARY_PATH man
Hi,
I am running a 64 bit Fedora 10 machine and having problems with coot, if i
say
ldd coot-real
linux-gate.so.1 => (0x5000)
libguilegtk-2.0.so.0 => not found
libgthread-2.0.so.0 => /lib/libgthread-2.0.so.0 (0x00df8000)
librt.so.1 => /lib/librt.so.1 (0x005f)
libguile.so
Jayashankar wrote:
I am still confused with what is right and wrong in this world of
research where the point of reference changes with time and condition.
Some body made me to accept that science and philosophy are verymuch
different?Are they ?
No,
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