Re: [ccp4bb] half occupancy question-further information

2008-10-18 Thread Ethan A Merritt
On Saturday 18 October 2008, Leoanrd M Thomas wrote: > To clarify a bit, the protein is not cyclic, it is two of the wild type > protein molecules joined together by a short peptide linker creating a > "new" protein. > > Here is my attempt at an ascii sketch, > > 2-fold

[ccp4bb] half occupancy question-further information

2008-10-18 Thread Leoanrd M Thomas
To clarify a bit, the protein is not cyclic, it is two of the wild type protein molecules joined together by a short peptide linker creating a "new" protein. Here is my attempt at an ascii sketch, 2-fold 2-fold __

Re: [ccp4bb] half occupancy linkage

2008-10-18 Thread Kay Diederichs
Leonard, I don't understand your description. - so this is indeed a cyclic protein, right? - Is it not the case that the two molecules per ASU in P32 are related by twofold NCS, and that in P3221 this is a crystallographic twofold? - What do you mean with "some percentage of the unit cells have