Re: [ccp4bb] Ammonium citrate tribasic buffer

2008-08-09 Thread William G. Scott
Hi Pete: The pKa (NH4OH) is about 9, so it shouldn't be relevant to the buffer calculation. The ammonia gas equilibrium would be a reason not to use this as a buffer, however. Bill William G. Scott Contact info: http://chemistry.ucsc.edu/~wgscott/ On Aug 9, 2008, at 2:54 PM, Dunten, P

Re: [ccp4bb] Ammonium citrate tribasic buffer

2008-08-09 Thread Dunten, Pete W.
Bill, You still lose a point for missing the fourth pKa in ammonium citrate, the ammonium ion. Kind regards, Pete -Original Message- From: CCP4 bulletin board on behalf of William G. Scott Sent: Sat 8/9/2008 12:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Ammonium citrate triba

Re: [ccp4bb] Ammonium citrate tribasic buffer

2008-08-09 Thread William G. Scott
Well, as long as I was right. That is the important thing. ;) William G. Scott Contact info: http://chemistry.ucsc.edu/~wgscott/ On Aug 9, 2008, at 11:11 AM, Ian Tickle wrote: I think the problem is that Britannica, or even Wikipedia, is and has never been the final arbiter of truth, I

Re: [ccp4bb] Ammonium citrate tribasic buffer

2008-08-09 Thread Ian Tickle
I think the problem is that Britannica, or even Wikipedia, is and has never been the final arbiter of truth, I would put my faith in a specialist publication such as: http://books.google.co.uk/books?id=bwbCVCFPNI4C&pg=PA175&lpg=PA175&dq=%22karl+hasselbalch%22&source=web&ots=yHzTUiNE1g&sig=7JmNU-