Well, I happen to lecture on this...
The bible is "Data for biochemical research", Dawson et al., Oxford
University Press.
Should be available in most biochemistry labs, if not in the library.
Cheers,
Nadir
--
Pr. Nadir T. Mrabet
Cellular & Molecular Biochemistry
INSERM U-724
Nancy
Tabled stability constants are e.g. in 'NIST Critically Selected
Stability Constants of Metal Complexes' an electronic database. I am
sure you find also tables in the library.
Another source is
http://old.iupac.org/publications/pac/1997/pdf/6907x1549.pdf; here
enthalpies are given for several
Dear all,
We have a position in the Crystallization group here at Abbott
Laboratories as listed below,
Those interested in applying should apply through the Abbott website
(www.abbott.com). Use the Job seekers link. The position can be found on
the site using the requisition number 56797BR.
Could those who responded with numbers for affinities of imidazole for metal
ions please divulge their sources? It is not that I doubt their veracities,
but it would be a nice reference to have on hand.
For those wondering about why I was asking about imidazole's affinity for
metal ions, I was
Dear all,
If anyone on this list has experience with the Fisher Scientific Isotemp
(model: 11-679-25CN) as a crystallization incubator, I would appreciate
any comments or recommendations. Many thanks in advance.
Sincerely,
thomas.
Hi Ed,
do you have an rule of thump estimating what imidazol concentration would
be bad?
I suppose 50 mM is not high enough to be bad. I once kept a protein at 4
deg C in 50 mM Imidazole/HCl, 5 mM NaN3, 1 mM DTT, pH 7.0). The protein
was fine for about half a year. Maybe I was very lucky.
Imidazole can indeed complex (monodentate) metal ions but not chelate
them (bidendate, at least).
However, the stability constant, K, of such complexes is rather low, eg
log K = 0.1 for Mg, 3.3 for Fe and 4.2 for Cu.
In comparison, metal chelates are formed with EDTA, for which log K =
10.6 for
Next MX-proposal application deadline: August 15th 2008
See:
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We kindly request MX-proposals for beamtime applications for the next
beamtime period.
In order to apply for beamtime, please register at the BESSY on-line
access tool "BOAT" (http://www.bessy.de/boat/www
Dear all, deadline for submission of proposals for beamtime at BM14 for
beamtime at ESRF during Aug/Sept 2008 is 25th July 2008 -get in your
proposals now before your summer holidays!. Beamline proposals can be
submitted on-line from the BM14 webpages (Click on Apply for Beamtime from
the MainMen
Hi,Philippe,
Have you tried to use Grasp or APBS in Pymol directly to do that? I
think they should work on the ions such Ca or others.
Good luck!
leo
Philippe Leone wrote:
Hi everybody,
I'd like to generate an electrostatic surface containing a Ca ion. I
tried to generate a pqr file to
I was wondering - why is imidazole bad for proteins?
I've always heard that high concentrations are "bad" for proteins, but a
bit of googling did not reveal the answer. Does it actually react with
side chains, or cause unfolding/aggragation?
Just wondering...
Good tip with the His-SELECT medi
Hi everybody,
I'd like to generate an electrostatic surface containing a Ca ion. I
tried to generate a pqr file to read it in Pymol, but the Ca is not
taken into account, so I tried to add directly the line correspondind to
the ion in the pqr file but it failed.
Any suggestion?
Philippe Leon
Dear Jacob,
this depends on type of metal ion, solvent accessibility of metal site
and pH.
E.g. the log of dissociation constants for histidine- Cu2+ / Ni2+/Zn2+
complexes are ca. 10.6, 8.7 and 6.6. Imidazol might pull out easily
these metals. Mg2+ and Ca2+ prefer coordination by oxygen. Thes
indeed!
Jane Wibley used the his-tag method for the preparation of human
O6-alkylguanine-DNA alkyltransferase, our "rivals" in California did not. They
found a (structural) Zn ion, we did not.
see pdbs 1qnt (Wibley et al. Nucleic Acids Res, 28, p393 (2000) and 1eh6
(Daniels et al., EMBO J 19
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