Dear All,
We are now accepting general user proposals for Run 3 2008 (September –
December) for beamline 3-BM1
The deadline for submission of proposals is July 1st 2008
The details on how to submit are outlined below:
Information of submission of proposals
http://www.synchrotron.org.au/co
Dear All
The PX group has a position open for a Scientific Support Officer.
We operate two PX beamlines at the Australian Synchrotron in Melbourne
Australia.
Information about the position can be found at:
http://www.synchrotron.org.au/content.asp?document_id=5390
I look forward to seeing
Mark et al.
Rfree can be calculated with as few as 150 reflections, depending on the
desired precision of Rfree.
The real issue is scaling. Many programs scale vs. the test set
independently, which becomes unstable with a small test set, and this is the
main cause of error in Rfree. The topic and
Mark J. van Raaij wrote:
On a related note, how to refine a structure with only 5000
reflections, which could happen when you have a small a.u. and modest
resolution? Could, exceptionally, a lower absolute amount of
reflections be used for Rfree, say 500?
Eleanor (mostly) answered my identical
Dear All,
It has recently been put to me that 5-10% of reflections should always
be set aside for calculation of Rfree. However, when one has, say, 200
000 reflections (high resolution and/or large asymmetric unit), that
seems to me to be a waste, because it would mean removing 10 000 to 20
Hi,
I had the same problem with my crystals because they grew as thin plates. My
precipitant was PEG8000, and the crystals grew thicker with addition of
glycerol or ethylene glycol.
Best,
Kianoush
--- On Mon, 6/16/08, shivesh kumar <[EMAIL PROTECTED]> wrote:
From: shivesh kumar <[EMAIL PROTEC
Several people have asked about the concentrations we use for various
dyes. In the case of fluorescein, we started with 45mg/ml as a stock
solution. This is quite arbitrary and we often found that our initial
stock solutions needed to be diluted 1:10 or 1:100 before use. It is
more art than
Shivesh
If you have not already, maybe try the following:
1. Adding other alcohols as an additive. See work by Keichi Namba in
improving his flagella crystals. The idea is to poison growth in one
direction. I have also had success in "thickening" crystals grown in MPD,
by addition of 1-3% isopro
Dear all,
I am trying to improve the quality of a xtal, crystallizing with MPD as a
precipitant at 16 degree C with thin plate like xtals. I would appreciate
suggestions to have diffraction quality xtals (thick). I am using proline,
glycine, glucose, sucrose as an additive.Thanx in advance.
shives
Whatever dye(s) you use, be sure to run some positive and negative
controls to see how the dye really works.
Jim
On Sat, 14 Jun 2008, Mark Del Campo wrote:
Before I place an order for some Izit, are there some other dyes I can
use to check if I've got a protein crystal?
Thanks,
Mark
A PhD student position in protein
crystallography / structural
biology is available in the
Laboratory for
Biocrystallography, Department of Pharmaceutical Sciences, University of Leuven, Belgium
(http://pharm.kuleuven.be/anafar/).
The PhD student will participate in one or more
high-impact
me
Dear All,
Just to remind you that the closing date for registration is now just
1 week away (22nd June).
Best wishes
Elspeth Garman (Chair) and Andrew Leslie (Vice Chair)
Gordon Research Conference on Diffraction Methods in Structural Biology
July 13-18, 2008, Bates College, Lewiston
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