Re: [ccp4bb] gfp problem

2008-06-02 Thread Artem Evdokimov
Hi, It soulds like you have serious disorder in the structure. On the subject of twinning - do you have the original diffraction images, and is there enough data to re-process in a lower space group? Sometimes it takes only a few non-symmetrical atoms to break an apparent higher symmetry, in which

[ccp4bb] gfp problem

2008-06-02 Thread Cui Sheng
Dear all: We have an unrefinable protein complex structure with one of the component is just a gfp. The space group shows as a P6122, a notorious space group for twining. But I could not detect any sign of twinnig using various of softwares. The R_free traps currently at 0.34 and no more space

Re: [ccp4bb] crystallisation

2008-06-02 Thread Roger Rowlett
sajid akthar wrote: Dear All My protein size is ~30kD and crystallizes with 19%Peg3350, 0.2M Nacl, and 0.1M Na Cacodylate buffer. Please refer the attached crystal image with this. The crystal looks like good enough for home source. These crystals appears in 4-5 days at room temp. Sometimes I'

[ccp4bb] EMBO Workshop Announcement

2008-06-02 Thread Kim Henrick
Announcement of an EMBO Workshop "Computational Aspects of the Protein Target Selection, Protein Production Management and Structure Analysis Pipeline" 22nd - 26th September 2008 To be held at EMBL Hinxton United Kingdom see http://www.ebi.ac.uk/msd-srv/docs/embo08/ for preliminary timetabl

Re: [ccp4bb] crystallisation

2008-06-02 Thread Navdeep Sidhu
What is the room temperature diffraction like? If that's good, you might like to optimize cryo. If I am not mistaken, PEG 3350 has been reported to cryoprotect at 20-35% with or without 5-10% glycerol. You might also like to try including some glycerol in the mother liquor--it may better crystal to

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread Nadir T. Mrabet
Hi, I bought myself a brand-new Viewsonic P227f crt less than a year ago (in France). So I suspected this item to be still available. But no longer!. I however found something that might still be of interest to you (http://www.viewsonic.com/products/crtmonitors/graphicseries/g90fB/). Seems to

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread Mike Latchem
These "new" TFT monitors do seem like a really good idea, but, it is well worth doing in depth research as most of the accessible (Cheap) ones designed for gaming currently only operate with directX. As I understand it there are plans to make these work with openGL as well as directX so they should

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread Jim Fairman
Hello all, There is a new company around that makes a 3D capable 22 inch flat screen LCD monitor (http://www.iz3d.com) capable of a maximum resolution of 1680x1050. It is originally designed for the purpose of playing video games in 3D, but looks promising for ridding ourselves of the huge bulky

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread Warren DeLano
For used-stereo-3D-capable-CRT seekers, our stereo 3D page may be of some use. A number of target monitors are listed. http://pymol.sourceforge.net/stereo3d.html -Original Message- From: CCP4 bulletin board on behalf of Krojer,Tobias Sent: Mon 6/2/2008 5:24 AM To: CCP4BB@JISCMAIL.AC.UK

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread David Roberts
Title: Message I've had good luck with refurbished monitors off the web.  Just do a google search for a sun monitor (for example) and it should come up.  Otherwise, go to pricewatch.com and search for crt monitors there.  They may have some external case dents, but the monitors are usually cris

Re: [ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread Dirk Kostrewa
Hi Tobias, we bought good, refurbished 22'' CRT monitors at eBay. Make sure to get monitors that have a horizontal refresh rate of about 130 kHz (or even higher), since this is usually the bottleneck. You need a horizontal refresh rate of ~123.5 kHz to display a stereo image with 1280x960

[ccp4bb] 3D model building without CRT monitor

2008-06-02 Thread Krojer,Tobias
Dear all, recently some of our CRT monitors broke down and we realized that these monitors are no longer produced. However, we would still like to continue model building in 3D which is apparently not possible with current TFT displays. Beside the possibility of getting old CRT monitors at ebay,

Re: [ccp4bb] DLS and SEC-LS facility

2008-06-02 Thread Raji Edayathumangalam
The facility at Yale University is very good. http://keck.med.yale.edu/biophysics/ Don't know about current wait times. I got excellent service. Good luck! Raji -Included Message-- >Date: 1-jun-2008 22:48:00 -0400 >From: "Michael Colaneri" <[EMAIL PROTECTED]> >To: >Subject:

[ccp4bb] Gordon Conference on Diffraction Methods in Structural Biology

2008-06-02 Thread Andrew Leslie
Dear All, Just to remind you that the closing date for registration is now just under 3 weeks away (22nd June). Best wishes Elspeth Garman (Chair) and Andrew Leslie (Vice Chair) Gordon Research Conference on Diffraction Methods in Structural Biology July 13-18, 2008, Bates College,