Hi,
It soulds like you have serious disorder in the structure. On the subject of
twinning - do you have the original diffraction images, and is there enough
data to re-process in a lower space group? Sometimes it takes only a few
non-symmetrical atoms to break an apparent higher symmetry, in which
Dear all:
We have an unrefinable protein complex structure with one of the component is
just a gfp. The space group shows as a P6122, a notorious space group for
twining. But I could not detect any sign of twinnig using various of softwares.
The R_free traps currently at 0.34 and no more space
sajid akthar wrote:
Dear All
My protein size is ~30kD and crystallizes with
19%Peg3350, 0.2M Nacl, and 0.1M Na Cacodylate buffer.
Please refer the attached crystal image with this. The
crystal looks like good enough for home source. These
crystals appears in 4-5 days at room temp.
Sometimes I'
Announcement of an EMBO Workshop
"Computational Aspects of the Protein Target
Selection, Protein Production Management and
Structure Analysis Pipeline"
22nd - 26th September 2008
To be held at EMBL Hinxton United Kingdom
see
http://www.ebi.ac.uk/msd-srv/docs/embo08/
for preliminary timetabl
What is the room temperature diffraction like? If that's good, you might like to
optimize cryo. If I am not mistaken, PEG 3350 has been reported to cryoprotect
at 20-35% with or without 5-10% glycerol. You might also like to try including
some glycerol in the mother liquor--it may better crystal to
Hi,
I bought myself a brand-new Viewsonic P227f crt less than a year ago (in
France).
So I suspected this item to be still available. But no longer!.
I however found something that might still be of interest to you
(http://www.viewsonic.com/products/crtmonitors/graphicseries/g90fB/).
Seems to
These "new" TFT monitors do seem like a really good idea, but, it is
well worth doing in depth research as most of the accessible (Cheap)
ones designed for gaming currently only operate with directX. As I
understand it there are plans to make these work with openGL as well as
directX so they should
Hello all,
There is a new company around that makes a 3D capable 22 inch flat screen
LCD monitor (http://www.iz3d.com) capable of a maximum resolution of
1680x1050. It is originally designed for the purpose of playing video games
in 3D, but looks promising for ridding ourselves of the huge bulky
For used-stereo-3D-capable-CRT seekers, our stereo 3D page may be of some use.
A number of target monitors are listed.
http://pymol.sourceforge.net/stereo3d.html
-Original Message-
From: CCP4 bulletin board on behalf of Krojer,Tobias
Sent: Mon 6/2/2008 5:24 AM
To: CCP4BB@JISCMAIL.AC.UK
Title: Message
I've had good luck with refurbished monitors off the web. Just do a
google search for a sun monitor (for example) and it should come up.
Otherwise, go to pricewatch.com and search for crt monitors there.
They may have some external case dents, but the monitors are usually
cris
Hi Tobias,
we bought good, refurbished 22'' CRT monitors at eBay. Make sure to
get monitors that have a horizontal refresh rate of about 130 kHz (or
even higher), since this is usually the bottleneck. You need a
horizontal refresh rate of ~123.5 kHz to display a stereo image with
1280x960
Dear all,
recently some of our CRT monitors broke down and we realized that these
monitors are no longer produced. However, we would still like to
continue model building in 3D which is apparently not possible with
current TFT displays. Beside the possibility of getting old CRT monitors
at ebay,
The facility at Yale University is very good.
http://keck.med.yale.edu/biophysics/
Don't know about current wait times. I got excellent service.
Good luck!
Raji
-Included Message--
>Date: 1-jun-2008 22:48:00 -0400
>From: "Michael Colaneri" <[EMAIL PROTECTED]>
>To:
>Subject:
Dear All,
Just to remind you that the closing date for registration is now just
under 3 weeks away (22nd June).
Best wishes
Elspeth Garman (Chair) and Andrew Leslie (Vice Chair)
Gordon Research Conference on Diffraction Methods in Structural Biology
July 13-18, 2008, Bates College,
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