[ccp4bb]

Hi, Can anyone direct me to a program that calculates a protein cavity's dimensions (average) and not just its volume? Thanks in advance, Xie

[ccp4bb] Postdoctoral opening at UT Southwestern

A postdoctoral position combining structural/biophysical techniques and protein engineering to discover the molecular mechanisms that control microtubule assembly is available immediately in Luke Rice’s lab in the Department of Biochemistry at UT Southwestern Medical Center. The lab is currentl

Re: [ccp4bb] odd waters

None except 8 Se. So either - a) L,K edge far below, or b) K-edge above 12.7keV. Cl fits a) and refines to B-factors close to the sourrounding protein atoms. Thx, br _ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Abhinav Kumar Sent: Tuesday, May 27, 2008 8:44 PM To: C

Re: [ccp4bb] insect expression system

Hi Yongfu, I couldn't agree more with Radu. We had great success in expressing mg amounts of a secreted protein in HEK293 cells (with Radu's help :-) . The same protein was initially expressed in Sf9 cells but with much lower yields. Furthermore, we could very easily generate a stable HEK29

Re: [ccp4bb] insect expression system

Dear Yong-fu, Why would you worry about insect expression systems if you already can secrete your constructs in mammalian cells? For such proteins, transient expression in HEK cells for example gives higher yields than baculo, is faster, cheaper, you can nicely control glycosylation, easily do

Re: [ccp4bb] insect expression system

Hi, It is called Bacmam. Please see the following reference, it is not the land mark reference, if you dig pubmed you should be able to find the first description of the system. BacMam recombinant baculovirus in transporter expression: a study of BCRP and OATP1B1. Protein Expr Purif. 2006 Jun;47(

[ccp4bb] BEAM TIME AVAILABLE FOR MACROMOLECULAR CRYSTALLOGRAPHY

BEAM TIME AVAILABLE FOR MACROMOLECULAR CRYSTALLOGRAPHY AT THE TWO ID BEAMLINES OF NE-CAT AT APS. Beamline 24ID-C: Variable energy between 6-18keV, ADSC Q315 Detector, MAD Capable. Beamline 24ID-E: Equipped with MD2 microdiffractometer, delivering beam as small as 5 microns. Single energy (12662e

Re: [ccp4bb] insect expression system

I searched my mail box for possible answers you have given but couldn't find any. The question is about selecting insect expression systems for mammalian or viral glycosylated proteins. There is confirmed expression of the target proteins in mammalian cells. They are secreted into the media.

Re: [ccp4bb] insect expression system

Hi, with S2 you can get away without figuring out if the virus works, then again you probably need a stable cell line... (so which ever works...?) (OBS! cant do SeMet labelling with S2!! or if someone can please tell me) -Tommi Quoting Yong-Fu Li <[EMAIL PROTECTED]>: > Hi, > > I searched my ma

[ccp4bb] insect expression system

Hi, I searched my mail box for possible answers you have given but couldn't find any. The question is about selecting insect expression systems for mammalian or viral glycosylated proteins. There is confirmed expression of the target proteins in mammalian cells. They are secreted into the media.

Re: [ccp4bb] Problem with crystallization of Se-Met labeled protein

Consider also the possibility that your SeMet protein differs from the native protein by something other than the sulfur --> selenium. Growing your cells in different conditions may induce different host proteins that could modify your protein. (Often we use rich media for native protein productio

Re: [ccp4bb] dry shipper on airplaine

Hi Clemens We normally check in the dewar as normal luggage after we have removed all liquids, we have never had any problems travelling from Aarhus to SLS. just remind the students coming along not to mention movies like Twelve monkeys or outbreak when checking it in. best Preben > Dear all, > >

[ccp4bb] Post-doctoral and PhD position : Structure-function of chemokine receptors

Post-doctoral and PhD position : Structure-function of chemokine receptors Corinne Vivès, Eva Pebay-Peyroula and Franck Fieschi, Institut de Biologie Structurale, UMR5075 CEA-CNRS-Univ. J.Fourier, 41, rue Jules Horowitz, F38027 Grenoble cedex 1, FRANCE In collaboration with Jean-Luc Popot, C.