[ccp4bb] Position in Protein Expression

2008-05-15 Thread Steve Almo
*Protein Expression Laboratory* A position is available immediately in the Macromolecular Therapeutics/Protein Production Facility at the Albert Einstein College of Medicine. This facility is housed within the new Center for Genetic and Translational Medicine at the Col

[ccp4bb] Position available in High Throughput Crystallization

2008-05-15 Thread Steve Almo
*Structural Genomics Crystallization* A technician position is now available at the Albert Einstein College of Medicine to participate in the the New York Structural Genomix Research Consortium (NYSGXRC; www.nysgxrc.org). The NYSGXRC is one of four NIH-funded high throu

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Meyer, Peter
Doesn't this just change the question to "what observation:parameter ratio is needed for my structure to be over-determined?"? As near as I'm aware, the answer to that one seems to be "as many observations as you get". Pete -Original Message- From: CCP4 bulletin board on behalf of [EMA

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread William Scott
On May 15, 2008, at 12:40 PM, Ed Pozharski wrote: I was just trying to protect poor creatures, after all they [hydrogens] only got one electron to hold on to (:-) Ed. A less radical view (pun intended) suggests two. ;)

[ccp4bb] Research Associate I/II position

2008-05-15 Thread Oganesyan, Vaheh
Colleagues, I'll appreciate if following advertisement will be brought to attention of qualified researchrs. An exciting research opportunity is available at MedImmune, Inc. (now part of AstraZeneca, Plc.) for a self motivated B.S. or M.S. level Scientist to contribute to ongoing projects that in

Re: [ccp4bb] UV light source for protein xtal detection

2008-05-15 Thread V. Nagarajan
We are about to release an all-LED based UV microscope for automated scanning of 96-well plates. Please contact me if you are interested. Thanks, V. Nagarajan JAN Scientific, Inc. Seattle, WA, USA. -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Li Zh

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Ed Pozharski
Sure, it has very little to do with original question about what constitutes "high" resolution. And that term is quite relative. 1.8A data is definitely of higher resolution than 2A, but is it "high"? (Not to mention the issue of subjective choice of resolution cutoff). The only way to define t

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread William Scott
The electrons on the hydrogens won't be centered about the proton nucleus, so if you want to resolve that as anything more than a bump, you will need a lot better than 1 Å (or neutrons). But in fairness the original question asked what you can call "high" resolution, not what you can call

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Ed Pozharski
Of course. However, C=0 bond is ~1.2A, and bonds made by those pesky hydrogens are ~1A. And I would think (it is semantics again) that to reach atomic resolution you have to resolve all atoms, otherwise "All atoms are equal, but some (non-hydrogens) are more equal than others." Cheers, Ed. O

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Bernhard Rupp
Optical reconstructions are intensity based. Xtallographic FT are amplitude based. The theoretical value for xray is about 0.9*dmin. Reading: Stenkamp, R.E. and L.H. Jensen. 1984 Resolution revisited. Acta Cryst., A40, 251-254. and the SFCHECK paper for other considerations Vaguine AA, Richelle J,

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Sue Roberts
I don't think the term "high resolution" has any real definition or meaning anymore. If you're proud of the resolution, put the number in the title of the paper and let the reader decide. At one time 2 A was high resolution, but I wouldn't consider that high resolution today for a plain v

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Bryan W. Lepore
On Thu, 15 May 2008, William Scott wrote: On May 15, 2008, at 10:01 AM, Ed Pozharski wrote: 1.2A (not surprisingly since this is about the length of covalent bond). A carbon-carbon single bond is about 1.55 Å. the van der Waals radius of hydrogen is 1.2A (Eisenberg/Crothers, Pauling, 1960 )

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread William Scott
Actually, if you want to feel really good, I learned (in the context of optics) that you can resolve two points separated by a distance x with a diffraction limit of x/0.7, so this means you can start to see carbon - carbon atomicity at 2.2 Å. On May 15, 2008, at 10:12 AM, Gloria Borgstahl w

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread William Scott
On May 15, 2008, at 10:01 AM, Ed Pozharski wrote: 1.2A (not surprisingly since this is about the length of covalent bond). A carbon-carbon single bond is about 1.55 Å.

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread Ed Pozharski
I don't think that individual atoms can be RESOLVED at 2A resolution. Even if we forget that hydrogen is also an atom, it is still true that peaks in electron density corresponding to, say, covalently bonded atoms are not separated. If atomic positions at 2A would be over-determined, we won't need

[ccp4bb] COURSE ANNOUNCEMENT - BIOCRYS 2008

2008-05-15 Thread Daniele de Sanctis
On behalf of the organizers, Maria Armenia Carrondo and Thomas R. Schneider COURSE ANNOUNCEMENT - BIOCRYS 2008 Fundamentals of Modern Methods in Biocrystallography - 'What you always wanted to know about crystallography but never dared to ask' http://biocrys.itqb.unl.pt 4th - 11th October

[ccp4bb] Crystallization position open at SGX Pharmaceuticals

2008-05-15 Thread Shane Atwell
Research Associate II, Crystallization CRYRA2-08 Research Associate II, Crystallization SGX Pharmaceuticals is an innovative San Diego-based biotechnology company focused on the discovery, development and commercialization of novel, targeted therapeutics directed at addressing unmet medical

Re: [ccp4bb] poll: cutoff for "high resolution"

2008-05-15 Thread proclus
On 14 May, Mark Del Campo wrote: > At what refinement resolution or resolution ranges would you call a structure > "high resolution" vs. > "low resolution"? I realize that this may boil down to semantics (e.g. some > may classify structures as > "medium resolution"), but I wanted to get an opi

Re: [ccp4bb] UV light source for protein xtal detection

2008-05-15 Thread Mark Agacan
I put an array of UV LEDS on a ceramic block and connected to a power source, as a means of detecting very small crystals once they are in the loop. The most difficult part was supporting the LED block so that it shone onto the loop but did not obstruct the user. The so-called UV LEDS are ofte

Re: [ccp4bb] UV light source for protein xtal detection

2008-05-15 Thread Li Zhijie
Hello, You may want to have a look at the UV LEDs, which should be the cheapest option if you only need a specific wavelenth. I found this on google: http://www.3dzled.com/other.html. It seems that they can make 280nM LEDs. It is interesting to note that they also said these LEDs' "Wavelengt

[ccp4bb] please post

2008-05-15 Thread Crina Nimigean
An exciting research opportunity in the ion channel structure and mechanism area is available for a highly motivated post-doctoral fellow at the Weill Medical College of Cornell University in New York City, Department of Anesthesiology. The research focuses on analyzing ion channel function wit

Re: [ccp4bb] UV light source for protein xtal detection

2008-05-15 Thread R.M. Garavito
Alfredo, It's not clear from your email what your purpose is: to illuminate a crystal/sample on a microscope stage or on a goniostat. In either case, you might check out Ocean Optics (http:// www.oceanoptics.com/). They have based their systems on fiber optic light sources and CCD detec

[ccp4bb] please post

2008-05-15 Thread Crina Nimigean
An exciting research opportunity in the ion channel structure and mechanism area is available for a highly motivated post-doctoral fellow at the Weill Medical College of Cornell University in New York City, Department of Anesthesiology. The research focuses on analyzing ion channel function wit

[ccp4bb] Industrial Liaison Scientist (MX) position at Diamond Light Source

2008-05-15 Thread Shotton, EJ (Elizabeth)
Dear All, I would like to draw your attention to the following position currently open at Diamond to establish and run a crystallography service for industrial users of the synchrotron. Regards Elizabeth =