Re: [ccp4bb] phasing at low resolution

2008-05-09 Thread Raja Dey
Hi Sajid, You should first check whether your Se sites are correct or not. I checked for my Se sites as follows: 1. Create predict_patterson map with se sites(obtained from shelx or solve) and see if that matches with Ano-diff patterson map. 2. Create SAD phase using se sites. Use tha

Re: [ccp4bb] phasing at low resolution

2008-05-09 Thread Tim Gruene
Hallo Sajid, shelxe produces both .phs file. This can be displayed in coot as electron density map. If you read in the .res file, it should show the 12 Se-sites. They are good guiding points to start model building. They should also help you judge whether your solution is at all usable and cor

[ccp4bb] phasing at low resolution

2008-05-09 Thread sajid akthar
Hi All I have a data set of Se-Met crystal 3.65A resolution collected at Inflection and peak wavelength. Also I have a data set at same resolution in native form. The protein size is 28000 dalton. I dont have any model for this protein. So I started to find the heavy atom peak search to find out

Re: [ccp4bb] How to generate two molecules of proteins and two chains of RNA in CNS 1.2

2008-05-09 Thread Stuart Endo-Streeter
Is this a copy-paste error in the e-mail text, or what is in actually the input file? {* separate chains by segid - a new segid starts a new chain *} {+ choice: true false +} {===>} n{= water files =} I do not see a value entered for t

Re: [ccp4bb] How to generate two molecules of proteins and two chains of RNA in CNS 1.2

2008-05-09 Thread Stuart Endo-Streeter
Does the log from generate show it reading in both RNA chains and do you have enough loading statements? Stuart Endo-Streeter On Thursday 08 May 2008 22:38, Sun Tang wrote: > Dear All, > > When I used CNS 1.2 to generate the pdb file for two molecules of > proteins and two chains of RNA (with

[ccp4bb] Call for proposals: SLS PX beamlines

2008-05-09 Thread heinz-josef . weyer
Dear SLS users, Call for Proposals: SLS PX Beamlines Call is open for proposals for the following crystallography beamlines of the Swiss Light Source, SLS: PXI (X06SA) PXII (X10SA) PXIII (X06DA) Peri

[ccp4bb] Bioxhit workshop in Heraklion

2008-05-09 Thread Evangelia Kapetaniou
Dear Community, this is an announcement for the approaching BIOXHIT course ‘From Crystals to Structures' on Crystallization, Diffraction Data Collection, Processing and Phasing at the Institute of Molecular Biology and Biotechnology, Heraklion, Greece from 23 – 25 June 2008 Lectures will be combi

[ccp4bb] Ninth Como meeting 29th September ­ 3r d October 2008

2008-05-09 Thread Derek Logan
REGISTRATION IS OPEN for the Ninth International School on the Crystallography of Biological Macromolecules Como (Italy), 29th September – 3rd October 2008 for more details and to register, please visit the web page: www.crystallographyschool.org ORGANIZERS Keith Wilson (York University, UK)

Re: [ccp4bb] HKL2000 and gcc4 - redux

2008-05-09 Thread Peter Keller
Hi, On Thu, 8 May 2008, James Stroud wrote: On May 7, 2008, at 3:07 PM, Chris Waddling wrote: so even temporarily putting a library where it doesn't belong Actually, this is what /usr/lib is for (except for the "doesn't" part). According to the Filesystem Hierarchy Standard 4.7 regarding