Hi Neeraj:
Terese Bergfors advises several things re PEG: buy gas chromatography quality,
don't mix brands/batches, store it in dark, +/- flush with nitrogen, store
aliquots at -20 deg C.
Reference: Terese Bergfors (ed), Protein Crystallization: Techniques,
Strategies, and Tips. A Laboratory Manu
Have a look at
Ray WJ Jr, Puvathingal JM.
A simple procedure for removing contaminating aldehydes and peroxides from
aqueous solutions of polyethylene glycols and of nonionic detergents that are
based on the polyoxyethylene linkage.
Anal Biochem. (1985) 146:307-12
http://dx.doi.org/10.1016/0003-
Hi alli have been trying to crystallize a protein and have had some
hits with conditions having PEG in them (400 and 4000).but everytime
i have tried to reproduce the conditions there has been a lot of
heterogeneity..and then to look for answers i delved a little deeper
into underst
Crystallization: Focus on Membrane Proteins
June 18-22, 2008
http://www.nsls.bnl.gov/newsroom/events/workshops/2008/crys/
A grant from The International Union of Crystallography will support the
attendance of a limited number of attendees. Preference will be given to Young
Scientists [graduate
Hi Everyone,
A number of postdoctoral positions are available at the Brookhaven National
Laboratory and the National Synchrotron Light Source (Long Island, NY, USA)
including one in Solution X-ray Scattering (JobID 14431 from the following
link).
https://jobs.bnl.gov/psc/Jobs/EMPLOYEE/HRMS/c
Dear colleagues,
please be informed that the registration slot for the
6th International NCCR Symposium on New Trends in Structural Biology
8 + 9 September 2008, University of Zürich, Lecture Hall KOH-B10,
Zürich, Switzerland
is now open. Online registration is possible directly from the symp
Hi Junyu
It is possible at 6 Å resolution, what I would try is to cut your data
and try searching in different resolution ranges 20-7 or 25-6.5 , if you
are uncertain about your search model, try with a higher RMS value try
going up to 2.0. If you have any data, even at very low resolution, wi
Dear all, deadline for submission of proposals for beamtime at BM14 for
beamtime at ESRF during June and July is 30th April 2008 -get in your
proposals now before the summer rush!. Beamline proposals can be submitted
on-line from the BM14 webpages (Click on Apply for Beamtime from the
MainMenu Fram
Call for proposals
for experiments at the macromolecular crystallography
beamlines at the Photon Factory, KEK, Japan
We are now accep
