Rajan Pillai wrote:
Can this problem arise due to loss of isomorphism in the crystals
after soaking with the substrate? The c-axis dimension increased by
~10 angstroms
Yes.
-James Holton
MAD Scientist
Hi All,
I want to confirm the binding of the substrate in the active site of the
enzyme by soaking crystals of the apo-protein with the substrate. I
generated a Fobs_lig - Fobs_apo difference electron density map. Inspection
of the map did not show the characteristic electron density of the
substr
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Dear Yolanda,
Here is the basic idea of prime-and-switch phasing:
We calculate phases from a model. These phases are mostly correct, but may
have incorrect peaks at the coordinates of some misplaced atoms in the
model.
Next we are going to go through our reflections one by one. We calculate
a m
Hi Thomas，Hi all,
I have used the amazing software "prime-and-switch" before,it is
quite helpful.
I have read through your article "Using prime-and-switch phasing to
reduce model bias in molecular replacement" , but it is hard for me to
understand. So what I am concerned is the basic
