just want to check my understanding on scala. the manual says :
"SdB may be omitted in the input and its use is not recommended. Default
values are 1.0, 0.0, 0.02"
the example shown is :
sdcorrection full 1.4 0.11 part 1.4 0.05
.. i.e. if SdB were set, it would have been between the 1.4 and
Vaheh,
I don't recall precipitation at all, but I do remember that we were prepared to
change the crystallization recipes (i.e. adjust the recipes from previous
'large volume' crystallization to make more nuclei). For example, our first
tests were with lysozyme (sorry, hardly a representative
The Structural Genomics Consortium (SGC) is adopting a biology-centered
approach to structural genomics: dedicated biology groups with
target-specific expertise work on getting the proteins soluble, and
biophysical and functional characterizations feature prominently.
Interesting biological questio
Actually, in our hands, we have gotten a mixture of clear drops and
precipitate when scaling from teeny drops to larger ones. Teeny drops
equilibrate faster, and have more pronounced surface and edge effects
than larger ones. We have also found that it is easier to crystallize
salt in teeny drops (
Surely the original poster meant "alternate indexing possibilites" (as
discussed in http://www.ccp4.ac.uk/dist/html/reindexing.html; for his
space group there are indeed 2 ways to index), not "alternate origins".
Kay
Meyer, Peter schrieb:
Doubtlessly I'm confused, but my understanding was tha
Doubtlessly I'm confused, but my understanding was that alternative origins
would only be an issue once a dataset was phased (so that wouldn't cause
problem during integrating/scaling). I'd thought that using a consistent unit
cell during indexing and scaling was a separate issue.
Any chance y
Dear F8 Cooters,
For the record, I'd recommend the stable release. You can even try the
Fedora-8 build:
http://www.ysbl.york.ac.uk/~emsley/software/binaries/stable/coot-0.4.1-binary-Linux-i386-fedora-8.tar.gz
Paul.
Petr Kolenko wrote:
Hi Fred,
we use this version and we did not have any p
Mark,
What was the state of the larger drops when tiny counterparts had
crystals? My guess - they all precipitated.
I'm trying to understand why some proteins or some conditions require
change in protein concentration while others do not when migrating from
smaller drops to larger ones. If it
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Not at the moment, but I'm open to suggestions!
Bryan W. Lepore wrote:
do any of fffear/pirate/buccaneer use SIGFP in a significant/meaningful
way?
-bryan
This illustrates nicely that the number of crystals per volume unit
ultimately depends on the number of nucleants per volume unit. If the
surface of your vessel has the power to nucleate it dramatically changes
the effect of going to smaller volumes. I guess everybody is right here,
depending on th
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